ATP Sulfurylase, S. cerevisiae Recombinant

• Catalog number
  P1542-20
• Price
  Please ask
• Size
  20 µg

• Synonyms
  Sulfate Adenylyltransferase, Met3, ATPS, Methionine-requiring protein 3, Sulfate adenylate transferase
• Alternative_names
  Sulfate Adenylyltransferase, Met3, ATPS, Methionine-requiring protein 3, Sulfate adenylate transferase
• Description
  A key enzyme used in pyrosequencing
• Recombinant
  1
• Source
  E. coli
• Purity by SDS PAGE
  >95%
• Assay
  SDS - PAGE
• Activity Specifications test method
  Enzyme activity assay: The activity of the enzyme is 100 U/mg. The enzyme’s activity was assayed using coupled hexokinase with BioVision’s PicoProbe™ Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Cat. No. K751).
• Biological activity
  Enzyme activity assay: The activity of the enzyme is 100 U/mg. The enzyme’s activity was assayed using coupled hexokinase with BioVision’s PicoProbe™ Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Cat. No. K751).
• Unit Definition
  One unit is the amount of enzyme that will catalyze 1 µmole of adenylyl sulfate (APS) to Adenosine Triphosphate (ATP) at pH 8.0 and 37 ̊C.
• Molecular Weight
  57.7 kDa (amino acids: 1-511 with N-terminal His-Tag)
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Lyophilized
• Reconstitution Instructions
  Reconstitute in 50 mM Tris pH 8, 30% glycerol, and 5 mM MgCl2 to a final concentration of 1 mg/ml.
• Background Information
  ATP Sulfurylase (EC No. EC2.7.7.4) catalyzes the first committed step in incorporating inorganic SO42- by converting ATP to adenylyl sulfate and pyrophosphate for the incorporation of inorganic SO42- into biomolecules. It is also a key enzyme used in pyrosequencing.
• Amino acid sequence
  The sequence is from Saccharomyces cerevisiae. Full-length ATP Sulfurylase (aa 1-511 with N-Terminal His-Tag) (Accession# P08536)
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  ATP   Sulfurylase   cerevisiae  
• Gene symbol
  VPS9D1, NFATC2
• Short name
  ATP Sulfurylase, S. cerevisiae Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  adenosine triphosphate Sulfurylase, S. cerevisiae Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:13526
• Gene
  VPS9D1
• Long gene name
  VPS9 domain containing 1
• Synonyms gene
  • C16orf7
• Synonyms gene name
  • chromosome 16 open reading frame 7
• Synonyms
  • ATP-BL
• GenBank acession
  • AB018551
• Locus
  16q24.3
• Discovery year
  2000-09-20
• Entrez gene record
  9605
• Pubmed identfication
  • 10231027
• RefSeq identity
  • NM_004913
• Classification
  • VPS9 domain containing
• VEGA ID
  OTTHUMG00000173219

Gene info

• Identity
  HGNC:7776
• Gene
  NFATC2
• Long gene name
  nuclear factor of activated T cells 2
• Synonyms gene name
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
  • nuclear factor of activated T-cells 2
• Synonyms
  • NF-ATP
  • NFATp
  • NFAT1
• GenBank acession
  • U43342
  • U43341
• Locus
  20q13.2
• Discovery year
  1994-11-16
• Entrez gene record
  4773
• Pubmed identfication
  • 8202141
• RefSeq identity
  • NM_012340
• Classification
  • IPT domain containing
  • Nuclear factors of activated T-cells
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000032747

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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