ATP Colorimetric/Fluorometric Assay Kit

• Catalog number
  K354-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly Sensitive, HTS
• Summary
  • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity- All • Applications- The assay can detect as low as 50 picomol (1 µM) of ATP in various samples.
• Sample Type
  Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Features Benefits
  • Simple procedure; takes less than 1 hour • Fast and convenient • Kit is designed to be a robust method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  ATP is the primary energy currency of living systems. Virtually all energy requiring processes utilize the chemical energy stored in the phosphate bond of ATP. ATP is formed exclusively in the mitochondria and a variety of genetic diseases can affect ATP formation in the mitochondria. There are a number of commercially available ATP assays which detects femtomoles or less of ATP by measuring luminescence (BioVision Kit 254-200, for example) but these kits require specialized luminescence instrumentation and utilize luciferase which can be difficult to maintain in active form. BioVision newly developed ATP Colorimetric and Fluorometric Assay kit is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (λmax = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect as low as 50 picomol (1 µM) of ATP in various samples. The kit provides sufficient reagents for 100 assays.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  ATP   Fluorometric   Kit  
• Gene symbol
  VPS9D1, NFATC2
• Short name
  ATP Colorimetric/Fluorometric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  adenosine triphosphate colorimetry/fluorescence test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:13526
• Gene
  VPS9D1
• Long gene name
  VPS9 domain containing 1
• Synonyms gene
  • C16orf7
• Synonyms gene name
  • chromosome 16 open reading frame 7
• Synonyms
  • ATP-BL
• GenBank acession
  • AB018551
• Locus
  16q24.3
• Discovery year
  2000-09-20
• Entrez gene record
  9605
• Pubmed identfication
  • 10231027
• RefSeq identity
  • NM_004913
• Classification
  • VPS9 domain containing
• VEGA ID
  OTTHUMG00000173219

Gene info

• Identity
  HGNC:7776
• Gene
  NFATC2
• Long gene name
  nuclear factor of activated T cells 2
• Synonyms gene name
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
  • nuclear factor of activated T-cells 2
• Synonyms
  • NF-ATP
  • NFATp
  • NFAT1
• GenBank acession
  • U43342
  • U43341
• Locus
  20q13.2
• Discovery year
  1994-11-16
• Entrez gene record
  4773
• Pubmed identfication
  • 8202141
• RefSeq identity
  • NM_012340
• Classification
  • IPT domain containing
  • Nuclear factors of activated T-cells
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000032747

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