Ascorbic Acid Colorimetric Assay Kit II (FRASC)
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Catalog number
K671-100
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Price
Please ask
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Size
100 assays
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Summary
• Detection method- Absorbance (545-600 nm) • Species reactivity- Mammalian • Application- The assay can detect 0.2 to 20 nmol of ascorbic acid in various samples.
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Sample Type
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth media and food products
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Features Benefits
• Simple procedure; takes ~ 30 minutes to complete the assay • Fast and convenient
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent and an immune stimulant and is present in a wide variety of biological specimens. Due to the presence of a variety of other antioxidants in biological samples such as serum, most ascorbic acid assays show strong interference. BioVision’s FRASC Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products. In this assay, Feᶟᶧ is reduced to Fe²ᶧ by any antioxidants present. The ferrous iron is chelated with a colorimetric probe to produce a product with a strong absorbance band which can be monitored between 545-600 nm. The addition of ascorbate oxidase to parallel samples removes any ascorbate present leaving a background value which is subtracted from the total to give ascorbate content. The assay can detect 0.2 to 20 nmol of ascorbic acid in various samples.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Short name
Ascorbic Acid Colorimetric Assay Kit II (FRASC)
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Ascorbic Acid colorimetry test reagent II (FRASC)
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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