Antibody/Protein Aggregation Assay Kit

  • Catalog number
    K308-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    Rapid & simple assay to measures total protein aggregation
  • Summary
    • Detection method- Fluorescence (Ex/Em = 440/500 nm) • Applications- Measurement of protein aggregation for protein formulation
  • Detection Method
    Detection method- Fluorescence (Ex/Em = 440/500 nm)
  • Species Reactivity
    N/A
  • Applications
    Measurement of protein aggregation for protein formulation
  • Features Benefits
    • Simple one-step procedure; takes less than one hour • High-throughput compatible • The entire assay can be performed directly in a 96-well plate and does not require washing/long incubation times or solubilization steps
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Antibody and protein aggregation are important issues in the field of Biotherapeutics. A wide range of conditions and different stages of processing of biopharmaceuticals, such as, formulation, purification, storage, shipping and handling, heat, freeze thaw cycles, mechanical stress, chemical treatment etc. might cause aggregation of proteins, enzymes and antibody targets. The possibility of protein aggregates in eliciting immunogenicity is a major concern for biotherapeutics, which might compromise the safety and efficacy of the biologically derived drug products. Recombinant protein production and storage would highly benefit from a high throughput screening method that can screen a variety of formulation and storage conditions of a particular target protein/antibody. Currently, different biophysical techniques such as Dynamic Light Scattering (DLS), Size-exclusion Chromatography with Multi-angle Light Scattering (SEC-MALS) can detect the presence of small amount of aggregates in a protein/antibody preparation, but they are not amenable for high-throughput screening format. BioVision’s Antibody/Protein Aggregation assay kit is a platform to estimate the extent of aggregation within a protein/antibody sample. This is a fast, high-throughput, plate-based fluorometric assay format and can detect as low as 0.5% antibody aggregate
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Test
    Biovision supplies other types of Assays as 1.
  • French translation
    anticorps
  • Gene target
  • Short name
    Antibody/Protein Aggregation Assay Kit
  • Technique
    aggregation, Antibody, Assay, antibodies against human proteins, antibodies for, assays
  • Host
    Assay
  • Alternative name
    (antibody to-)/Protein Aggregation test reagent
  • Alternative technique
    antibodies, arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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