Anti-Histone H2AX Rabbit Monoclonal Antibody

• Catalog number
  A1143-25
• Price
  Please ask
• Size
  25 µg

• Antibody Target
  H2AFX
• Target Alternative Name
  H3F3A, H3.3A, H3F3, H3F3B, H3.3B
• Antibody Type
  Monoclonal
• Clone
  RM214
• Species Reactivities
  Human
• Immunogen Sequence
  A peptide corresponding to the C-terminus of human Histone H2AX
• Accession
  P16104
• Gene Id
  3014
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  1 mg/ml
• Formulation
  In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
• Purification
  Affinity purified using protein A
• Application
  WB, ICC, ELISA, Multiplex
• Positive Control
  WB: A375, HEK293, HeLa and SK-MEL-2; ICC: HeLa cells
• Application And Usages
  Western Blot: 0.5 ug/mL – 2 ug/mL; ICC: 1 ug/mL - 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.2 ug/mL – 1 ug/mL.
• Description
  Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

• Gene target
  Histone   H2AX   Monoclonal  
• Gene symbol
  H2AX
• Short name
  Anti-Histone H2AX Rabbit Monoclonal Antibody
• Technique
  Antibody, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Rabbit, Rabbits
• Isotype
  Rabbit IgG
• Alternative name
  Antibody toHistone H2AX production species: rabbit monoclonal (antibody to-)
• Alternative technique
  antibodies, rabbit-anti

Gene info

• Identity
  HGNC:4739
• Gene
  H2AX
• Long gene name
  H2A.X variant histone
• Synonyms gene
  • H2AFX
• Synonyms gene name
  • H2A histone family, member X
  • H2A histone family member X
• GenBank acession
  • X14850
• Locus
  11q23.3
• Discovery year
  1994-11-22
• Entrez gene record
  3014
• Pubmed identfication
  • 8076949
• RefSeq identity
  • NM_002105
• Classification
  • H2A histones
• VEGA ID
  OTTHUMG00000166196

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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