Anti-Histone H2A Rabbit Monoclonal Antibody

  • Catalog number
    A1145-25
  • Price
    Please ask
  • Size
    25 µg
  • Antibody Target
    HIST1H2AB
  • Target Alternative Name
    Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m
  • Antibody Type
    Monoclonal
  • Clone
    RM225
  • Species Reactivities
    Human
  • Immunogen Sequence
    A peptide corresponding to the C-terminus of human Histone H2A
  • Accession
    P04908, P0C0S8, P20671
  • Gene Id
    3012
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Concentration
    1 mg/ml
  • Formulation
    In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
  • Purification
    Affinity purified using protein A
  • Application
    WB, ICC, ELISA, Multiplex
  • Positive Control
    WB: HeLa, A375, SK-MEL-2, A431, and K562 cell lyastes; ICC: HeLa cells
  • Application And Usages
    WB: 0.5 µg/mL - 2 µg/mL; ICC: 1 µg/mL - 2 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.2 µg/mL - 1 µg/mL.
  • Description
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • FACS Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Dot Blot Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Additional description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • French translation
    anticorps
  • Gene target
  • Gene symbol
    H2AC20, H2AC8, H2AC7, H2AC18, H2AC17, H2AC11, H2AC19, H2AC15, H2AC4, H2AC14
  • Short name
    Anti-Histone H2A Rabbit Monoclonal Antibody
  • Technique
    Antibody, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
  • Host
    Rabbit, Rabbits
  • Isotype
    Rabbit IgG
  • Alternative name
    Antibody toHistone H2A production species: rabbit monoclonal (antibody to-)
  • Alternative technique
    antibodies, rabbit-anti
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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