Anti-H3R8me1 Antibody
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Catalog numberA2045-100
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PricePlease ask
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Size100 µl
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Antibody TargetH3R8me1
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Target Alternative NameH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
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Antibody TypePolyclonal
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Species ReactivitiesRat, Mouse, Human
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Immunogen SequenceA synthetic methylated peptide targeting residues around Arginine 8 of human Histone H3
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AccessionQ16695
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Gene Id8290
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AppearanceColorless liquid
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FormLiquid
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FormulationIn PBS with 0.02% sodium azide, 50% glycerol, pH 7.3
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PurificationAffinity purified
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ApplicationImmunohistochemistry (IHC), Immunofluorescence (IF), Dot Blot, Western Blotting
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Application And UsagesWB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200
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DescriptionHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack poly A tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
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Storage Condition-20ºC
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Shipping ConditionGel Pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedWB data verified in HeLa cells and H3 protein
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Immunocytochemistry VerifiedTrue
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Immunofluorescence VerifiedTrue
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Immunoprecipitation VerifiedFalse
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FACS VerifiedFalse
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ELISA VerifiedFalse
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ChIP VerifiedFalse
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Dot Blot VerifiedTrue
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Flow Cytometry VerifiedFalse
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Additional descriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Short nameAnti-H3R8me1 Antibody
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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IsotypeIgG
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Alternative nameAntibody toH3R8me1 (antibody to-)
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data