Anti-GPT/ALT1 Antibody

• Catalog number
  A1271-100
• Price
  Please ask
• Size
  100 µl

• Antibody Target
  GPT
• Target Alternative Name
  Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, GPT 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT, AAT1, GPT1
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, Mouse, Rat
• Immunogen Sequence
  KLH conjugated synthetic peptide between 118-144 amino acids from the N-terminal region of human GPT
• Accession
  P24298
• Gene Id
  2875
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS with 0.09% (W/V) sodium azide
• Purification
  Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Application
  IHC, WB
• Positive Control
  IHC: human liver and kidney tissue sections; WB: U87- mg cell line, mouse liver and rat heart and liver tissue lysates
• Application And Usages
  IHC: 1:100; WB: 1:1000
• Description
  GPT(Glutamate-pyruvate transaminase), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. This protein has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2). See ALTQTL1 and ALTQTL2 for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.
• Storage Condition
  -20°C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  GPT/ALT1  
• Gene symbol
  GPT, DPAGT1
• Short name
  Anti-GPT/ALT1 Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibody toGPT/ALT1 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:4552
• Gene
  GPT
• Long gene name
  glutamic--pyruvic transaminase
• Synonyms gene name
  • glutamic-pyruvate transaminase (alanine aminotransferase)
• Synonyms
  • ALT1
  • GPT1
• Synonyms name
  • alanine aminotransferase
  • alanine transaminase
• Locus
  8q24.3
• Discovery year
  2001-06-22
• Entrez gene record
  2875
• VEGA ID
  OTTHUMG00000165176

Gene info

• Identity
  HGNC:2995
• Gene
  DPAGT1
• Long gene name
  dolichyl-phosphate N-acetylglucosaminephosphotransferase 1
• Synonyms gene
  • DPAGT2
  • DPAGT
• Synonyms gene name
  • dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)
• Synonyms
  • GPT
  • D11S366
  • DGPT
  • ALG7
  • CDG-Ij
• Synonyms name
  • GlcNAc-1-P transferase 1
  • UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase 1
• GenBank acession
  • Z82022
• Locus
  11q23.3
• Discovery year
  1993-12-13
• Entrez gene record
  1798
• Pubmed identfication
  • 8244387
• RefSeq identity
  • NM_001382
• VEGA ID
  OTTHUMG00000153533
• Locus Specific Databases
  • Congenital Disorders of Glycosylation pages

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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