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Synonyms
1,4-alpha-D-Glucan-glucanohydrolase, ptyalin
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Alternative_names
1,4-alpha-D-Glucan-glucanohydrolase, ptyalin
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Description
An enzyme that breaks starch down into sugar
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Recombinant
False
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Source
Human Saliva
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Purity by SDS PAGE
> 90%
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Assay
SDS-PAGE.
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Activity Specifications test method
> 400 U/mg protein
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Unit Definition
One unit will catalyze the hydrolysis of one micromole 2-chloro-4-nitrophenyl-a-D-maltotrioside to yield 2-chloro-4-nitrophenol per minute at 37°C.
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Molecular Weight
~60,000
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Storage Temp
-20ºC
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Shipping
Gel Pack
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Shelf Life
12 months
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Concentration
> 0.2 mg protein/mg (Coomassie)
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Appearance
Lyophilized
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Physical form description
Lyophilized
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Reconstitution Instructions
Reconstitute in PBS at ≥ 100 units/mL. Further dilutions should be in the lyophilization buffer (75 mM sodium chloride, 10 mM tris chloride, 1 mM calcium chloride, pH 7.2) or a similar buffer. Calcium is important for the best activity and stability.
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Background Information
Amylase is an enzyme that breaks starch down into sugar. Amylase is present in human saliva, where it begins the chemical process of digestion. Foods that contain much starch but little sugar, such as rice and potato, taste slightly sweet as they are chewed because amylase turns some of their starch into sugar in the mouth. Alpha-Amylase is an enzyme that hydrolyses alpha-bonds of large alpha-linked polysaccharides such as starch and glycogen, yielding glucose and maltose. It is being used by researchers as a barometer of the body's response to physical or psychological stress.
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Handling
Centrifuge the vial prior to opening.
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Additional description
The Alpha-Amylase, Saliva is a α- or alpha protein sometimes glycoprotein present in blood.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
