Alkaline Phosphatase Staining Kit

• Catalog number
  K2035-50
• Price
  Please ask
• Size
  50 assays

• Description
  To identify and screen Alkaline Phosphatase activity in cells
• Summary
  The Alkaline Phosphatase (AP) Staining Kit provides a quick and easy method to stain Alkaline Phosphatase in pluripotent stem cells. It is based on the ability of Alkaline Phosphatase to hydrolyze the phosphate group on the substrate, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) to form a blue colored intermediate. Nitro Blue Tetrazolium (NBT) then oxidizes the intermediate to form an insoluble, dark blue dimer. Because stem cells/pluripotent stem cells have increased AP activity, the AP Staining Kit can be used to identify and screen Alkaline Phosphatase activity in these cells. The staining method is simple, rapid, cost-effective and reliable. The entire procedure can be performed in the same microtiter plate and does not require any extra steps such as fixing or harvesting the cells. The assay can be also used as the initial step to test the pluripotency of cells and to monitor osteogenic differentiation of pluripotent/stem cells.
• Detection Method
  Staining followed by microscopy
• Species Reactivity
  All
• Applications
  AP Staining in stem cells/pluripotent stem cells or stem cells undergoing osteogenesis., Screening for pluripotent cells induced by various factors., Detection of alkaline phosphate activity in stem cells/pluripotent stem cells, HeLa, HepG2, HEK293 cells, osteoblasts and osteocytes.
• Sample Type
  Induced pluripotent stem cells, Stem cells, Cell lines containing Alkaline Phosphatase activity
• Features Benefits
  To identify and screen Alkaline Phosphatase activity in cells, Rapid, Cost-effective, Reliable, Simple
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  The Alkaline Phosphatase (AP) Staining Kit provides a quick and easy method to stain Alkaline Phosphatase in pluripotent stem cells. It is based on the ability of Alkaline Phosphatase to hydrolyze the phosphate group on the substrate, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) to form a blue colored intermediate. Nitro Blue Tetrazolium (NBT) then oxidizes the intermediate to form an insoluble, dark blue dimer. Because stem cells/pluripotent stem cells have increased AP activity, the AP Staining Kit can be used to identify and screen Alkaline Phosphatase activity in these cells. The staining method is simple, rapid, cost-effective and reliable. The entire procedure can be performed in the same microtiter plate and does not require any extra steps such as fixing or harvesting the cells. The assay can be also used as the initial step to test the pluripotency of cells and to monitor osteogenic differentiation of pluripotent/stem cells.
• Data Sheet Link

• Gene target
  Alkaline   Phosphatase   Staining   Kit  
• Short name
  Alkaline Phosphatase Staining Kit
• Label
  Alkaline Phosphatase
• Alternative name
  Alkaline Phosphatase coloring reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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