AITRL, Human Recombinant

• Catalog number
  7113-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  Activation-induced TNFR member Ligand, TNFSF18, GITRL, TL-6
• Alternative_names
  Activation-induced TNFR member Ligand, TNFSF18, GITRL, TL-6
• Description
  Regulator of T-cell proliferation and survival, and involved in the interaction between T lymphocytes and endothelial cells
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 0.1 ng/ug of protein (
• Activity Specifications test method
  Determined by its ability to stimulate IL-8 production by human PBMC using a concentration range of 1.0-10.0 ng/ml. Note: Results may vary with PBMC donors.
• Biological activity
  Determined by its ability to stimulate IL-8 production by human PBMC using a concentration range of 1.0-10.0 ng/ml. Note: Results may vary with PBMC donors.
• Molecular Weight
  14.4 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Tris, pH 8.0.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Background Information
  AITRL, a member of the TNF superfamily, is expressed in endothelial cells, and signals through the AITR receptor. AITRL regulates T-cell proliferation and survival, and effectuates the interaction between T lymphocytes and endothelial cells. The AITRL gene codes for a type II transmembrane protein comprised of 177 amino acids, including a 28 amino acid cytoplasmic region, a 21 amino acid transmembrane domain and a 128 amino acid extracellular domain. Recombinant human soluble AITRL is a 14.4 kDa protein, containing 127 amino acid residues corresponding to the extracellular domain of AITRL.
• Amino acid sequence
  METAKEPCMA KFGPLPSKWQ MASSEPPCVN KVSDWKLEIL QNGLYLIYGQ VAPNANYNDV APFEVRLYKN KDMIQTLTNK SKIQNVGGTY ELHVGDTIDL IFNSEHQVLK NNTYWGIILL ANPQFIS
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  AITRL  
• Gene symbol
  TNFSF18
• Short name
  AITRL, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  AITRL, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:11932
• Gene
  TNFSF18
• Long gene name
  TNF superfamily member 18
• Synonyms gene name
  • tumor necrosis factor (ligand) superfamily, member 18
• Synonyms
  • AITRL
  • TL6
  • hGITRL
• GenBank acession
  • AF117713
• Locus
  1q25.1
• Discovery year
  1999-01-15
• Entrez gene record
  8995
• Pubmed identfication
  • 10037686
  • 10074428
• RefSeq identity
  • NM_005092
• Classification
  • Tumor necrosis factor superfamily
• VEGA ID
  OTTHUMG00000034835

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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