AHCY Inhibitor Screening Kit (Fluorometric)
-
Catalog number
K326-100
-
Price
Please ask
-
Size
100 assays
-
-
Description
Simple assay for high-throughput screening of AHCY Inhibitors
-
Summary
• Detection method- Fluorescence (Ex/Em = 392/482 nm) • Application-Screening/characterizing/studying AHCY inhibitors
-
Detection Method
Fluorescence (Ex/Em = 392/482 nm)
-
Applications
Screening/characterizing/studying AHCY inhibitors
-
Features Benefits
• Simple and reliable test to screen AHCY inhibitors • High-throughput suitable • Includes Inhibitor Control (3-Deazaneplanocin A)
-
Storage Conditions
-20°C
-
Shipping Conditions
gel pack
-
Shelf life
12 months
-
Background
Adenosyl homocysteinase (AHCY) (EC 3.3.1.1) or S-adenosyl homocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. Inhibition of AHCY results in the accumulation of SAH, a product inhibitor of S-adenosyl methionine (SAM)-dependent methyltransferases. AHCY is an important target as an antiviral and anticancer drug. Several characterized SAH inhibitors inhibit some DNA viruses (e.g. Pox viruses) and some negative stranded RNA viruses (e.g.: Marburg virus, Ebola virus & rabies). In BioVision’s AHCY Inhibitor Screening kit the homocysteine generated from the breakdown of SAH is measured using a Thiol Detecting Reagent resulting in enhanced fluorescence which is measured at Ex/Em = 392/482 nm. The AHCY reaction is reversible and hence Adenosine Deaminase is included in the reaction to ensure that the reaction proceeds towards hydrolysis of SAH. In the presence of AHCY inhibitor, there is a decrease in fluorescence of the Thiol Detecting Reagent.
-
-
Additional description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
-
Gene target
-
Gene symbol
AHCY
-
Short name
AHCY Inhibitor Screening Kit (Fluorometric)
-
Alternative name
AHCY suppressor detection reagent (fluorescence)
-
Alternative technique
kits
-
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
-
Gene info
-
Identity
-
Gene
-
Long gene name
adenosylhomocysteinase
-
Synonyms gene name
- S-adenosylhomocysteine hydrolase
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year
1986-01-01
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Product images
Similar products
