Adipogenesis Colorimetric/Fluorometric Assay Kit

  • Catalog number
    K610-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    Highly Sensitive Assay
  • Summary
    • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian • Application- The kit can detect triglyceride in as few as a thousand or less differentiated 3T3-L1 cells with triglyceride detection linear range 0.2 to 10 nmol.
  • Sample Type
    Adipocyte precursors such as 3T3 cells, human preadipocytes etc.
  • Features Benefits
    • Simple procedure; takes ~40 minutes • Fast and convenient • Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipogenesis in various samples.
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured. BioVision’s adipogenesis assay kit quantifies triglyceride accumulation in cells and tissues. In the assay, triglycerides are efficiently solubilized then hydrolyzed to glycerol which is subsequently oxidized to convert the probe to generate color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect triglyceride in as few as a thousand or less differentiated 3T3-L1 cells with triglyceride detection linear range 0.2 to 10 nmol. The high detection sensitivity and the convenient microplate assay format make the kit a convenient tool for studying the effect of adipogenesis inducers or inhibitors, or to screen drugs.
  • Test
    Biovision supplies other types of Assays as 1.
  • Properties
    Colorimetric assays or detection use UV absorption or enzymatic color reaction.
  • Gene target
  • Short name
    Adipogenesis Colorimetric/Fluorometric Assay Kit
  • Technique
    colorimetric, Assay, assays, colorimetry
  • Host
    Assay
  • Alternative name
    Adipogenesis colorimetry/fluorescence test reagent
  • Alternative technique
    assays, arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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