Active HRV14 3C Protease Recombinant
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Catalog number
8012-100
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Price
Please ask
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Size
100 µg
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Synonyms
Precission protease, 3C Protease
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Alternative_names
Precission protease, 3C Protease
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Description
≥90% Pure Active Recombinant HRV14 3C Protease for inhibitor screening assays, activity studies, selectivity profiling, western blotting, ELISA, and numerous such applications.
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥ 90%
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Assay
SDS-PAGE
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Activity Specifications test method
BioVision’s Active HRV14 3C Protease has been tested for its activity using HRV 3C Protease Activity Assay Kit (Colorimetric) (K214-100).
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Biological activity
BioVision’s Active HRV14 3C Protease has been tested for its activity using HRV 3C Protease Activity Assay Kit (Colorimetric) (K214-100).
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Results
BioVision’s Active HRV14 3C Protease has been tested for its activity using HRV 3C Protease Activity Assay Kit (Colorimetric) (K214-100).
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Molecular Weight
20 kDa (1538-1719 aa).
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Storage Temp
-80°C
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Shipping
Dry ice
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Shelf Life
6 months
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Concentration
1 mg/ml
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Appearance
Liquid
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Physical form description
1 mg/ml in 50 mM Tris, 100 mM NaCl, 5 mM DTT, pH 8.0 containing 20% glycerol
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Background Information
Human rhinovirus (HRV) infections are the most frequent causative agents of common cold and various other upper respiratory tract infections. Rhinoviruses are members of the picornavirus family, which have a positive-sense, single-stranded RNA genome that is translated into a single polyprotein precursor. In the case of HRVs, the viral polyprotein is mainly processed by the proteases (2A and 3C) to generate functional proteins and enzymes. BioVision’s active HRV14 3C Protease is a recombinant form of the 3C protease from human rhinovirus type 14. The enzyme is highly pure with selective and high site-specific cleavage activity for Precission cleavage site (Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro), thereby allowing for the production of recombinant proteins with minimal number of extra amino acids. HRV14 3C Protease also has therapeutic implications as a potential target for the development of antiviral agents.
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Amino acid sequence
1538-1719 aa
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
Active HRV14 3C Protease Recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
functionnal HRV14 3C Protease Rec.
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Alternative technique
rec
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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