Activated Protein C (APC) Inhibitor Screening Kit (Colorimetric)
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Catalog number
K427-100
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Price
Please ask
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Size
100 assays
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Description
High-throughput compatible, quick and easy screening for Activated Protein C ligands; Includes human recombinant APC
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Summary
• Detection method - Colorimetric (OD 405 nm) • Species reactivity - N/A • Applications - Screening of drugs and novel ligands for interaction with human recombinant Activated Protein C
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Detection Method
Detection method - Colorimetric (OD 405 nm)
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Species Reactivity
N/A
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Applications
Screening of drugs and novel ligands for interaction with human recombinant Activated Protein C
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Sample Type
Samples containing drugs, inhibitors or ligands (compounds that can interact and affect APC activity)
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Features Benefits
• Simple, highly sensitive, high-throughput compatible • Rapid screening of Activated Protein C ligands • Kit includes PPACK (APC inhibitor) and a stable, recombinant human APC
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Activated protein C (APC) (3.4.21.69), a Vitamin K dependent serine protease, is formed by activation of Protein C by Thrombomodulin bound on the surface of endothelial cells. By degrading coagulation factors Va and VIIIa, APC inhibits blood coagulation which helps in the prevention of thrombosis. Blood coagulation can be stimulated by inhibition of APC. Thus, APC inhibitors play an important role in the treatment of disease like Haemophilia. BioVision’s APC Inhibitor Screening Kit can be used to screen for potent inhibitors of APC activity. It utilizes the proteolytic activity of an active APC on a peptide substrate which releases a colored product. The released product can be easily quantified using an absorbance microplate reader. In the presence of an APC inhibitor, the enzyme decreases/loses activity resulting in lower/no absorbance. This assay kit is simple and can be used to identify and characterize APC inhibitors in a high-throughput format. As a control, we provide the inhibitor PPACK dihydrochloride - a known APC inhibitor.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Additional description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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Gene target
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Gene symbol
APC
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Short name
Activated Protein C (APC) Inhibitor Screening Kit (Colorimetric)
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Technique
colorimetric, colorimetry
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Label
APC
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Alternative name
Activated Protein C (adenomatous polyposis coli) suppressor detection reagent (colorimetry)
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Alternative technique
assays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Product images
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