Acetylcholinesterase Inhibitor Screening Kit (Colorimetric)

• Catalog number
  K197-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly Sensitive Assay
• Summary
  • Detection: Absorbance (OD 412 nm) • Application: - Screening/characterizing Acetylcholinesterase inhibitors
• Detection Method
  Absorbance (OD 412 nm)
• Applications
  • Screening of AChE inhibitors
• Features Benefits
  • Rapid, simple & convenient • This assay kit can screen potential human acetylcholinesterase inhibitors
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Acetylcholinesterase (AChE; EC 3.1.1.7) is the main cholinesterase enzyme in the nervous system. It is responsible for the hydrolysis of acetylcholine and some other choline esters that function as neurotransmitters. AChE is highly expressed in both the brain and the peripheral nervous system. It is especially enriched at neuromuscular junctions, where its activity serves to terminate synaptic transmission between motor neurons and skeletal muscles. Reversible inhibitors of AChE (such as donepezil, rivastigmine and galantamine) are commonly used in the treatment of Alzheimer’s Disease and other neurodegenerative disorders. However, irreversible inhibitors of AChE tend to be extremely poisonous, causing muscular paralysis, convulsions, bronchial constriction and eventual death by asphyxia. BioVision’s Acetylcholinesterase Inhibitor Screening Kit can be used to screen for potential inhibitors of AChE activity. It utilizes the ability of an active human AChE enzyme to hydrolyze the provided colorimetric substrate, generating a yellow chromophore that can be detected by measuring absorbance at 412 nm. In the presence of the potent reversible AChE inhibitor donepezil, enzyme activity is suppressed, preventing chromophore generation. The assay kit is adapted to a 96-well format and provides a rapid, simple and reliable test for high-throughput screening of AChE inhibitors
• Data Sheet Link
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  Acetylcholinesterase   Inhibitor   Screening   Kit  
• Short name
  Acetylcholinesterase Inhibitor Screening Kit (Colorimetric)
• Technique
  colorimetric, colorimetry
• Alternative name
  Acetylcholinesterase suppressor detection reagent (colorimetry)
• Alternative technique
  assays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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