Recombinant Streptavidin Streptavidin lyophilized powder
-
Catalog number
10 110 010
-
Price
Please ask
-
Size
1 mg lyophilized
-
-
ELISA category
Streptavidin lyophilized powder
-
ELISA detection limits
N/A
-
ELISA sensitivity
N/A
-
ELISA s description
Streptavidin is a tetrameric protein with a molecular weight of approximately 53 kD consisting of four subunits of approximately 13 kD, each containing a single biotin-binding site. It binds very tightly to the small molecule Biotin. Recombinant Streptavidin, produced by bacteria of the species Streptomyces avidinii, is expressed in E. coli. It is provided as a lyophilized product. Streptavidin coatings of solid phases offer a universal immobilization principle for the detection and analysis of proteins, peptides, PCR-fragments, haptens etc., which must be present in biotinylated form. In comparison to the direct coating method it avoids or minimizes denaturation of the biomolecules.
-
Research area interests
all immuno assays
-
Package form
lyophilized
-
Antigen
Biotin
-
Reacts with
Biotin
-
Tested applications
Use Streptavidin, recombinant for coating of solid phases (e.g., microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system). For life science research only. Not for use in diagnostic procedures.
-
Specificity
Biotin
-
Crossreactivity
N/A
-
Preservative materials
If applicable mentioned in the product data sheet
-
Storage recommendations
12 month
-
Shipping and storage conditions
ice pack
-
Long term storage
2-8°C
-
Assay principle
Streptavidin is a coating compound to immobilize biotinylated molecules
-
Included in the standard package
N/A
-
Not included in the standard package
N/A
-
Samples to be analyzed
coating material: microplates, beads, membranes etc. made Nitrocellulose, ceramics or plastics like Polystyrene, Polyethylene, Polypropylene, Polycarbonate, Poly(methyl methacrylate) PMMA or Polyamides
-
PubMed citations
See the datasheet
-
Technical datasheet
Contact us to receive the datasheet
-
Representative figures
No Available
-
Warnings
For Research Use only.
-
-
About
The term “freeze-dry” is a lay description for the process of lyophilization protein powder. It is the conversion of water from a frozen state to a gaseous state without going through a liquid state. Aliquoting with 25 mM HEPES-KOH, pH 7.2 and 1 mM DTT and 10% weight on volume glycerol is suitable for storage of the reconstituted powder. The freeze-dry process removes moisture from the cells of specimens while the specimens remain frozen.
-
Source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Short name
Recombinant Streptavidin Streptavidin powder
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. biotez advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Label
streptavidin
-
Alternative name
Rec. biotinilated-binding streptavidin biotinilated-binding streptavidin lyophilized powder
-
Alternative technique
rec
-
MeSH Data
-
Name
-
Concept
Scope note:
Method of tissue preparation in which the tissue specimen is frozen and then dehydrated at low temperature in a high vacuum. This method is also used for dehydrating pharmaceutical and food products.
-
Tree numbers
- E01.370.225.500.620.760.160.260
- E01.370.225.750.600.760.160.260
- E02.792.156.260
- E05.200.500.620.760.160.260
- E05.200.750.600.760.160.260
- E05.760.156.260
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products