Recombinant Streptavidin Streptavidin lyophilized powder

  • Catalog number
    10 110 010
  • Price
    Please ask
  • Size
    1 mg lyophilized
  • ELISA category
    Streptavidin lyophilized powder
  • ELISA detection limits
    N/A
  • ELISA sensitivity
    N/A
  • ELISA s description
    Streptavidin is a tetrameric protein with a molecular weight of approximately 53 kD consisting of four subunits of approximately 13 kD, each containing a single biotin-binding site. It binds very tightly to the small molecule Biotin. Recombinant Streptavidin, produced by bacteria of the species Streptomyces avidinii, is expressed in E. coli. It is provided as a lyophilized product. Streptavidin coatings of solid phases offer a universal immobilization principle for the detection and analysis of proteins, peptides, PCR-fragments, haptens etc., which must be present in biotinylated form. In comparison to the direct coating method it avoids or minimizes denaturation of the biomolecules.
  • Research area interests
    all immuno assays
  • Package form
    lyophilized
  • Antigen
    Biotin
  • Reacts with
    Biotin
  • Tested applications
    Use Streptavidin, recombinant for coating of solid phases (e.g., microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system). For life science research only. Not for use in diagnostic procedures.
  • Specificity
    Biotin
  • Crossreactivity
    N/A
  • Preservative materials
    If applicable mentioned in the product data sheet
  • Storage recommendations
    12 month
  • Shipping and storage conditions
    ice pack
  • Long term storage
    2-8°C
  • Assay principle
    Streptavidin is a coating compound to immobilize biotinylated molecules
  • Included in the standard package
    N/A
  • Not included in the standard package
    N/A
  • Samples to be analyzed
    coating material: microplates, beads, membranes etc. made Nitrocellulose, ceramics or plastics like Polystyrene, Polyethylene, Polypropylene, Polycarbonate, Poly(methyl methacrylate) PMMA or Polyamides
  • PubMed citations
    See the datasheet
  • Technical datasheet
    Contact us to receive the datasheet
  • Representative figures
    No Available
  • Warnings
    For Research Use only.
  • About
    The term “freeze-dry” is a lay description for the process of lyophilization protein powder. It is the conversion of water from a frozen state to a gaseous state without going through a liquid state. Aliquoting with 25 mM HEPES-KOH, pH 7.2 and 1 mM DTT and 10% weight on volume glycerol is suitable for storage of the reconstituted powder. The freeze-dry process removes moisture from the cells of specimens while the specimens remain frozen.
  • Source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Short name
    Recombinant Streptavidin Streptavidin powder
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. biotez advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Label
    streptavidin
  • Alternative name
    Rec. biotinilated-binding streptavidin biotinilated-binding streptavidin lyophilized powder
  • Alternative technique
    rec
MeSH Data
  • Name
  • Concept
    Scope note: Method of tissue preparation in which the tissue specimen is frozen and then dehydrated at low temperature in a high vacuum. This method is also used for dehydrating pharmaceutical and food products.
  • Tree numbers
    • E01.370.225.500.620.760.160.260
    • E01.370.225.750.600.760.160.260
    • E02.792.156.260
    • E05.200.500.620.760.160.260
    • E05.200.750.600.760.160.260
    • E05.760.156.260
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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