HtrA1 mini Extraction & Western Blot Kit
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Catalog number30 501 103
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Price:
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Size10 extraction & 10 blots
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ELISA categoryExtraction & Western Blot Kit
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ELISA detection limits0.05 - 1 µg/ extract
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ELISA sensitivity30 - 40 ng/µl Beads (bindungs-capacity)
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ELISA s descriptionEach kit provides sufficient reagents for preparing 10 extracts for an enrichment of HtrA1 about 200-300 ng per extract and for 10 detection of human HtrA1 with Western Blot.
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Research area interestsCancer, Rheumatic Diseases, Arthritis, Cardiovascular, Cell Biology, Signal Transduction, Immunology
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Package formsingle tube
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AntigenHtrA1
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Reacts withHtrA1
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Tested applicationsThis package provides the EXTRACT mini HtrA1 kit together with a high specific monoclonal antibody against human HtrA1 full length and a recombinant HtrA1 protein control for Western Blot.
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SpecificityHtrA1
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CrossreactivityN/A
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Preservative materialsIf applicable mentioned in the product data sheet
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Storage recommendations6 month
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Shipping and storage conditionsice pack
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Long term storage-20°C and 2-8°C
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Assay principlespecific Affinity chromatography and Western Blot
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Included in the standard packageantibody coupled beads, buffers, filters, control protein, primary antibody
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Not included in the standard packageSpectrophotometer, Pipettes or pipetting equipment, Disposable polypropylene test tubes, Distilled or deionised water
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Samples to be analyzedbiological fluids, tissues, cell culture
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PubMed citationsSee the datasheet
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Technical datasheetContact us to receive the datasheet
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Representative figures
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WarningsFor Research Use only.
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TestWestern Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
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Gene target
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Short nameHtrA1 mini Extraction & Blot Kit
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TechniqueWestern Blot, Blot, western Blots
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Alternative nameHtrA serine peptidase 1 mini Extraction & Western Blot reagent
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Alternative techniquewestern, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data