-
Long name
TrkA (Tyr680 + Tyr681)/TrkB (Tyr706 + Tyr707) Antibody
-
Also known as
TrkA (Tyr680 + Tyr681)/TrkB (Tyr706 + Tyr707) Antibody
-
Category
Primary Antibodies
-
Conjugation
Unconjugated
-
Target Antigen
TrkA Tyr680 + Tyr681, TrkB Tyr706 + Tyr707
-
Specificity
This is a highly specific antibody against TrkA Tyr680 + Tyr681, TrkB Tyr706 + Tyr707. The phosphorylation sites Tyr680 and Tyr681 in TrkA are homologous to those of Tyr683 and Tyr683 in Mouse and Rat. These sites in TrkB are homologous to those of Tyr705 and Tyr706 in Mouse and Rat.
-
Modification s
Phosphorylation
-
Modification site s
Tyr680+ Tyr681
-
Clonality
Polyclonal antibody
-
Clone number
Polyclonal antibody
-
Concentration
1ug per 1ul
-
Immunogen range
670-720/822
-
Subcellular location
Cell membrane
-
Source
KLH conjugated synthetic phosphopeptide derived from human TrkB around the phosphorylation site of Tyr680/681
-
Gene ID number
4914
-
Swiss Prot
P04629
-
Tested Applications
WB, IHC-P, IF(IHC-P)
-
Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
-
Cross reactivity
Human, Mouse, Rat
-
Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Background of the target antigen
Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors. Isoform TrkA-III is resistant to NGF, constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.
-
Purification method
This antibody was purified via Protein A.
-
Storage conditions
Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
-
Synonym names
MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A; gp140trk; NTRK1
-
Properties
If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translation
anticorps