TOP1 (1291CT875.142.166) Monoclonal Antibody

• Catalog number
  bsm-51396M
• Price
  Please ask
• Size
  0.1ml

• Long name
  TOP1 (1291CT875.142.166) Monoclonal Antibody
• Also known as
  TOP1 (1291CT875.142.166) Monoclonal Antibody
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  TOP1
• Specificity
  This is a highly specific antibody against TOP1.
• Modification s
  None
• Modification site s
  Unmodified antibody
• Clonality
  Monoclonal
• Clone number
  1291CT875.142.166
• Source
  This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.
• Gene ID number
  7150
• Swiss Prot
  P11387
• Tested Applications
  WB, FCM
• Recommended dilutions
  WB(1:1000), FC(1:25),
• Cross reactivity
  Human, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the target antigen
  Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
• Purification method
  Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Synonym names
  TOPI; DNA topoisomerase 1; DNA topoisomerase I; TOP1
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  TOP1   1291CT875   142   166   Monoclonal  
• Gene symbol
  TOP1, PLCG1-AS1
• Short name
  Anti-TOP1
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  Immunoglobulin G (IgG)1 κ
• Alternative name
  TOP1 (1291CT875.142.166) Monoclonal Antibody
• Alternative technique
  antibodies
• Alternative to gene target
  topoisomerase (DNA) I, TOPI, TOP1 and IDBG-75139 and ENSG00000198900 and 7150, poly(A) RNA binding, nuclei, Top1 and IDBG-211615 and ENSMUSG00000070544 and 21969, BT.39255 and IDBG-642660 and ENSBTAG00000007960 and 534799

Gene info

• Identity
  HGNC:11986
• Gene
  TOP1
• Long gene name
  DNA topoisomerase I
• Synonyms gene name
  • topoisomerase (DNA) I
• Synonyms name
  • Scl-70 antigen
• Locus
  20q12
• Discovery year
  1989-02-23
• Entrez gene record
  7150
• Classification
  • Topoisomerases
• VEGA ID
  OTTHUMG00000033057

Gene info

• Identity
  HGNC:40450
• Gene
  PLCG1-AS1
• Long gene name
  PLCG1 antisense RNA 1
• Synonyms
  • TOP1-AS1
• Locus
  20q12
• Discovery year
  2014-06-13
• Entrez gene record
  101927117
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000032476

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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