SIAH1 Antibody

• Catalog number
  bs-3596R
• Price
  Please ask
• Size
  0.1ml

• Long name
  SIAH1 Primary Polyclonal Antibody
• Also known as
  SIAH1 PAb
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  SIAH1
• Specificity
  This is a highly specific antibody against SIAH1.
• Modification s
  None
• Modification site s
  Unmodified antibody
• Clonality
  Polyclonal antibody
• Clone number
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen range
  200-250/282
• Subcellular location
  Cytoplasm, Nucleus
• Source
  This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human SIAH1
• Gene ID number
  6477
• Swiss Prot
  Q8IUQ4
• Tested Applications
  WB, IHC-P, IF(IHC-P)
• Recommended dilutions
  WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
• Cross reactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the target antigen
  E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Synonym names
  SIAH1A; E3 ubiquitin-protein ligase SIAH1; Seven in absentia homolog 1; Siah-1; Siah-1a; SIAH1; HUMSIAH
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  SIAH1  
• Gene symbol
  SIAH1
• Short name
  Anti-SIAH1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Host
  Rabbit (Oryctolagus cuniculus)
• Isotype
  Immunoglobulin G (IgG)
• Alternative name
  SIAH1 Polyclonal Antibody
• Alternative technique
  antibodies
• Alternative to gene target
  siah E3 ubiquitin protein ligase 1, SIAH1A, SIAH1 and IDBG-30112 and ENSG00000196470 and 6477, ligase activity, nuclei, BT.37798 and IDBG-631496 and ENSBTAG00000010868 and 534655

Gene info

• Identity
  HGNC:10857
• Gene
  SIAH1
• Long gene name
  siah E3 ubiquitin protein ligase 1
• Synonyms gene name
  • seven in absentia homolog 1 (Drosophila)
• Synonyms
  • hSIAH1
• GenBank acession
  • U76247
• Locus
  16q12.1
• Discovery year
  1997-09-12
• Entrez gene record
  6477
• Pubmed identfication
  • 9403064
  • 9334332
• Classification
  • Ring finger proteins
• VEGA ID
  OTTHUMG00000175417

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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