MYPT1 (Ser668) Antibody
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Catalog numberbs-3286R
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PricePlease ask
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Size0.1ml
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Long nameMYPT1 (Ser668) Primary Polyclonal Antibody
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Also known asMYPT1 (Ser668) PAb
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CategoryPrimary Antibodies
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ConjugationUnconjugated
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Target AntigenMYPT1 Ser668
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SpecificityThis is a highly specific antibody against MYPT1 Ser668.
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Modification sPhosphorylation
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Modification site sSer668
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ClonalityPolyclonal antibody
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Clone numberPolyclonal antibody
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Concentration1ug per 1ul
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SourceKLH conjugated synthetic phosphopeptide derived from human around the phosphorylation site of Ser668
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Gene ID number4659
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Tested ApplicationsWB, IHC-P, IF(IHC-P)
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Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
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Cross reactivityHuman, Mouse, Rat
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Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigenMyosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in non muscle cells. The guanosine triphosphate (GTP) bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho associated kinase (Rho kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Therefore Rho appears to inhibit myosin phosphatase through the action of Rho kinase.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym namesMYPT1 Ser668; Myosin Phosphatase phospho Ser668; Myosin Phosphatase phospho S668; p-MYPT1Ser668; Myosin Phosphatase; M130; MBS; MGC133042; Myosin phosphatase target subunit 1; Myosin phosphatase targeting subunit 1; MYPT 1; MYPT1; PPP1R12A; Protein phosphatase 1 regulatory inhibitor subunit 12A; Protein phosphatase 1 regulatory subunit 12A; Protein phosphatase myosin binding subunit; MYPT1_HUMAN; Myosin phosphatase-targeting subunit 1; Protein phosphatase myosin-binding subunit.
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PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolPPP1R12A
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Short nameAnti-MYPT1 Ser668
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TechniqueAntibody, antibodies against human proteins, antibodies for
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HostRabbit (Oryctolagus cuniculus)
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IsotypeImmunoglobulin G (IgG)
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Alternative nameMYPT1 (Ser668) Polyclonal Antibody
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameprotein phosphatase 1 regulatory subunit 12A
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Synonyms gene
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Synonyms gene name
- protein phosphatase 1, regulatory (inhibitor) subunit 12A
- protein phosphatase 1, regulatory subunit 12A
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-12-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ankyrin repeat domain containing
- Myosin phosphatase targeting family
- Protein phosphatase 1 regulatory subunits
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data