Human IgG (3E8) Monoclonal Antibody

• Catalog number
  bsm-50220M
• Price
  Please ask
• Size
  0.1ml

• Long name
  Human IgG (3E8) Monoclonal Antibody
• Also known as
  Human IgG (3E8) Monoclonal Antibody
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  Human IgG
• Specificity
  This is a highly specific antibody against Human IgG.
• Modification s
  None
• Modification site s
  Unmodified antibody
• Clonality
  Monoclonal
• Clone number
  300000000
• Concentration
  1ug per 1ul
• Source
  Protein purified from Human plasma
• Swiss Prot
  P01857
• Tested Applications
  WB, IHC-P, IP
• Recommended dilutions
  WB (0.1ug/mL) IP (1.0ug) IHC-P
• Cross reactivity
  Human
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the target antigen
  Immunoglobulin G (IgG) is a monomeric immunoglobulin, built of two heavy chains γ and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids. This is the only isotype that can pass through the placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). 1) IgG1, IgG3 and IgG4 cross the placenta easily. 2) IgG3 is the most effective complement activator and IgG4 does not activate complement. 3) IgG1 and IgG3 bind with high affinity to Fc receptors on phagocytic cells. IgG4 has intermediate affinity and IgG2 affinity is extremely low.
• Purification method
  Purified by Protein G.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  3E8   Monoclonal  
• Short name
  Anti-Human IgG
• Technique
  Antibody, IgG, antibodies against human proteins, antibodies for, IgGs, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  Immunoglobulin G (IgG)1 κ, IgG
• Species
  Human, Humans
• Alternative name
  Human IgG (3E8) Monoclonal Antibody
• Alternative technique
  antibodies, immunoglobulins

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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