HSF1(Ser303) Antibody
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Catalog numberbs-5363R
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PricePlease ask
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Size0.1ml
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Long nameHSF1(Ser303) Primary Polyclonal Antibody
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Also known asHSF1(Ser303) PAb
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CategoryPrimary Antibodies
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ConjugationUnconjugated
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Target AntigenHSF1 Ser303
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SpecificityThis is a highly specific antibody against HSF1 Ser303.
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Modification sPhosphorylation
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Modification site sSer303
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ClonalityPolyclonal antibody
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Clone numberPolyclonal antibody
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Concentration1ug per 1ul
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SourceKLH conjugated synthetic phosphopeptide derived from human HSF1 around the phosphorylation site of Ser303
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Gene ID number3297
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Tested ApplicationsWB, IHC-P, IF(IHC-P)
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Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
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Cross reactivityHuman, Mouse, Rat
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Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigenTwo HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells.HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym namesHSF1Ser303; Heat shock factor 1; Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; hsf1; HSTF 1; HSTF1.
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PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolHSF1
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Short nameAnti-HSF1 Ser303
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TechniqueAntibody, antibodies against human proteins, antibodies for
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HostRabbit (Oryctolagus cuniculus)
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IsotypeImmunoglobulin G (IgG)
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Alternative nameHSF1(Ser303) Polyclonal Antibody
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Alternative techniqueantibodies
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Alternative to gene targetheat shock transcription factor 1, HSTF1, HSF1 and IDBG-40498 and ENSG00000185122 and 3297, sequence-specific DNA binding, nuclei, Hsf1 and IDBG-155311 and ENSMUSG00000022556 and 15499, HSF1 and IDBG-643592 and ENSBTAG00000020751 and 506235
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Gene info
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Identity
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Gene
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Long gene nameheat shock transcription factor 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-09-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data