GFP (18A11) Monoclonal Antibody
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Catalog number
bsm-50187M
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Price
Please ask
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Size
0.1ml
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Long name
GFP (18A11) Monoclonal Antibody
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Also known as
GFP (18A11) Monoclonal Antibody
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Category
Primary Antibodies
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Conjugation
Unconjugated
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Target Antigen
GFP
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Specificity
This is a highly specific antibody against GFP.
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Modification s
None
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Modification site s
Unmodified antibody
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Clonality
Monoclonal
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Clone number
18A11
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Source
Recombinant green fluorescent protein
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Swiss Prot
P42212
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Tested Applications
IP
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Recommended dilutions
IP (1 ul)
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigen
Green fluorescent protein (GFP) isolated from jellyfish Aequorea aequorea is a 238 amino acid protein with an apparent molecular weight of about 27-30 kDa on SDS-PAGE. Its chromophore is formed by cyclisation and oxidation of the three amino acids Ser65, Tyr66, and Gly67. The numerous applications include : using GFP as a reporter for gene expression, as a marker to study cell lineage during development and as a tag to localize proteins in living cells. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology.
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Purification method
Ammonium sulfate precipitation
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Storage conditions
Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Properties
If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
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Gene target
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Short name
Anti-GFP
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Technique
Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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Host
Mouse (Mus musculus)
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Isotype
Immunoglobulin G (IgG)1 κ
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Label
gfp
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Alternative name
GFP (18A11) Monoclonal Antibody
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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