E3 ubiquitin ligase Antibody
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Catalog number
bs-9663R
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Price
Please ask
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Size
0.1ml
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Long name
E3 ubiquitin ligase Primary Polyclonal Antibody
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Also known as
E3 ubiquitin ligase PAb
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Category
Primary Antibodies
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Conjugation
Unconjugated
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Target Antigen
E3 ubiquitin ligase
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Specificity
This is a highly specific antibody against E3 ubiquitin ligase.
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Modification s
None
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Modification site s
Unmodified antibody
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Clonality
Polyclonal antibody
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Clone number
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen range
45-95/352
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Subcellular location
Mitochondrion
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Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human E3 ubiquitin ligase/RNF218
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Gene ID number
79594
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Swiss Prot
Q969V5
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Tested Applications
WB, IHC-P, IF(IHC-P)
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Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
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Cross reactivity
Human, Mouse, Rat
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigen
Exhibits weak E3 ubiquitin-protein ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Can ubiquitinate AKT1 preferentially at 'Lys-284' involving 'Lys-48'-linked polyubiquitination and seems to be involved in regulation of Akt signaling by targeting phosphorylated Akt to proteosomal degradation. Proposed to preferentially act as a SUMO E3 ligase at physiological concentrations. Plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. The function may implicate its abilty to sumoylate DNM1L. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. Involved in the modulation of innate immune defense against viruses by inhibiting DDX58-dependent antiviral response. Can mediate DDX58 sumoylation and disrupt its polyubiquitination.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym names
GIDE; MAPL; MULAN; RNF218; C1orf166; Mitochondrial ubiquitin ligase activator of NFKB 1; E3 SUMO-protein ligase MUL1; E3 ubiquitin-protein ligase MUL1; Growth inhibition and death E3 ligase; Mitochondrial-anchored protein ligase; Putative NF-kappa-B-activating protein 266; RING finger protein 218; MUL1
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Properties
If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
ITCH, HECTD3, PRKN
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Short name
Anti-E3 ubiquitin ligase
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Technique
Antibody, antibodies against human proteins, antibodies for
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Host
Rabbit (Oryctolagus cuniculus)
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Isotype
Immunoglobulin G (IgG)
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Alternative name
E3 ubiquitin ligase Polyclonal Antibody
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Alternative technique
antibodies
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Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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