DNase I Antibody
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Catalog numberbs-7651R
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PricePlease ask
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Size0.1ml
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Long nameDNase I Primary Polyclonal Antibody
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Also known asDNase I PAb
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CategoryPrimary Antibodies
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ConjugationUnconjugated
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Target AntigenDNase I
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SpecificityThis is a highly specific antibody against DNase I.
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Modification sNone
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Modification site sUnmodified antibody
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ClonalityPolyclonal antibody
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Clone numberPolyclonal antibody
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Concentration1ug per 1ul
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SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human DNase I
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Gene ID number1773
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Tested ApplicationsWB, IHC-P, IF(IHC-P)
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Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
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Cross reactivityHuman, Mouse, Rat
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Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigenDeoxyribonuclease I gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA; it was the first DNase to be crystallized; and it was the first DNase for which a specific protein inhibitor was characterized. DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym namesRNASE1; Dornase alfa; Deoxyribonuclease 1; Deoxyribonuclease I; Deoxyribonuclease1; DeoxyribonucleaseI; DNASE 1; DNase I lysosomal; DNASE1; DNaseI; DNL 1; DNL1; DRNI; Human urine deoxyribonuclease I; DNAS1_HUMAN.
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PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolDNASE1L3, DNASE1L1, SET
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Short nameAnti-DNase I
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TechniqueAntibody, antibodies against human proteins, antibodies for
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HostRabbit (Oryctolagus cuniculus)
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IsotypeImmunoglobulin G (IgG)
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Alternative nameDNase I Polyclonal Antibody
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namedeoxyribonuclease 1 like 3
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Synonyms gene name
- deoxyribonuclease I like 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-05-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namedeoxyribonuclease 1 like 1
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Synonyms gene
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Synonyms gene name
- deoxyribonuclease I-like 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-05-09
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Entrez gene record
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Pubmed identfication
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameSET nuclear proto-oncogene
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Synonyms gene name
- SET translocation (myeloid leukemia-associated)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-04-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- INHAT complex
- SET complex
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data