BIG1/ARFGEF1 Antibody
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Catalog numberbs-12866R
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PricePlease ask
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Size0.1ml
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Long nameBIG1/ARFGEF1 Primary Polyclonal Antibody
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Also known asBIG1/ARFGEF1 PAb
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CategoryPrimary Antibodies
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ConjugationUnconjugated
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Target AntigenBIG1/ARFGEF1
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SpecificityThis is a highly specific antibody against BIG1/ARFGEF1.
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Modification sNone
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Modification site sUnmodified antibody
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ClonalityPolyclonal antibody
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Clone numberPolyclonal antibody
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Concentration1ug per 1ul
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SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human BIG1/ARFGEF1
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Gene ID number10565
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Tested ApplicationsWB, IHC-P, IF(IHC-P)
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Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
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Cross reactivityHuman, Mouse, Rat
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Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigenGuanine nucleotide-exchange proteins (GEPs) accelerate replacement of bound GDP with GTP and thereby activate ADP-ribosylation factors (ARFs), a family of guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking. GEPs comprise two major families, large GEPs that are inhibited by brefeldin A (BFA), a protein that effects Golgi structure and a group of smaller GEPs that are insenstive to BFA. Two genes for GEPs found on human chromosomes 8 and 20 encode BFA sensitive GEPs designated BIG1 and BIG2. Both GEPS contain a sec7 domain that is responsible for their brefeldin inhibition and also their catalytic activity. In vivo, BIG1 and BIG2 exist in macromolecular complexes that move between the Golgi membranes and cytosol. BIG2 associates with PKA regulatory subunits, implying that BIG2 may act as an A kinase-anchoring protein (AKAP) that could coordinate the cAMP and ARF regulatory pathways.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym namesADPribosylation factor guanine nucleotide exchange factor 1brefeldin A inhibited; ARFGEF1; ARFGEP1; Brefeldin A inhibited GEP 1; Brefeldin A inhibited guanine nucleotide exchange protein 1; p200 ARF GEP1; p200 ARF guanine nucleotide exchange factor; BIG1_HUMAN.
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PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolARFGEF1, ARFGEF1-DT
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Short nameAnti-BIG1/ARFGEF1
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TechniqueAntibody, antibodies against human proteins, antibodies for
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HostRabbit (Oryctolagus cuniculus)
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IsotypeImmunoglobulin G (IgG)
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Alternative nameBIG1/ARFGEF1 Polyclonal Antibody
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Alternative techniqueantibodies
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Alternative to gene targetADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited), ARFGEP1 and BIG1 and P200, ARFGEF1 and IDBG-24344 and ENSG00000066777 and 10565, protein kinase A regulatory subunit binding, nuclei, Arfgef1 and IDBG-133303 and ENSMUSG00000067851 and 211673, ARFGEF1 and IDBG-644666 and ENSBTAG00000014691 and 281022
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Gene info
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Identity
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Gene
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Long gene nameADP ribosylation factor guanine nucleotide exchange factor 1
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Synonyms gene name
- ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-08-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ARFGEF family
- Armadillo like helical domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameARFGEF1 divergent transcript
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Locus
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Discovery year2020-09-16
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Entrez gene record
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RefSeq identity
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Classification
- Divergent transcripts
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data