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Long name
alpha2 Adaptin Primary Polyclonal Antibody
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Also known as
alpha2 Adaptin PAb
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Category
Primary Antibodies
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Conjugation
Unconjugated
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Target Antigen
alpha2 Adaptin
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Specificity
This is a highly specific antibody against alpha2 Adaptin. Based on the similarity among the Alpha2-Adaptin proteins, this antibody will bind both AP2A1 and AP2A2.
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Modification s
None
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Modification site s
Unmodified antibody
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Clonality
Polyclonal antibody
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Clone number
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen range
25-75/939
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Subcellular location
Cell membrane
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Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human AP2A2
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Gene ID number
161
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Tested Applications
WB, IHC-P, IF(IHC-P)
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Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
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Cross reactivity
Human, Mouse, Rat
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigen
Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans-Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha-adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym names
AP-2 complex subunit alpha-2; 100 kDa coated vesicle protein C; Adaptor protein complex AP-2 subunit alpha-2; Adaptor-related protein complex 2 subunit alpha-2; Alpha-adaptin C; Alpha2-adaptin; Clathrin assembly protein complex 2 alpha-C large chain; Huntingtin yeast partner J; Huntingtin-interacting protein 9; HIP-9; Huntingtin-interacting protein J; Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit; AP2A2; ADTAB; CLAPA2; AP-2 complex subunit alpha-1; 100 kDa coated vesicle protein A; Adaptor protein complex AP-2 subunit alpha-1; Adaptor-related protein complex 2 subunit alpha-1; Alpha-adaptin A; Alpha1-adaptin; Clathrin assembly protein complex 2 alpha-A large chain; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit; AP2A1; ADTAA; CLAPA1
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Properties
If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
