ZAP70 (Tyr493) Antibody, FITC Conjugated
-
Catalog number
bs-3479R-FITC
-
Price
Please ask
-
Size
0.1ml
-
-
Long name
ZAP70 (Tyr493) Polyclonal Antibody, FITC Conjugated
-
Category
Conjugated Primary Antibodies
-
Conjugation
FITC
-
Host Organism
Rabbit (Oryctolagus cuniculus)
-
Target Antigen
ZAP70 Tyr493
-
Specificity
This is a highly specific antibody against ZAP70 Tyr493.
-
Modification
Phosphorylation
-
Modification site
Tyr493
-
Clonality
Polyclonal
-
Clone
Polyclonal antibody
-
Concentration
1ug per 1ul
-
Source
KLH conjugated synthetic phosphopeptide derived from human Zap-70 around the phosphorylation site of Tyr493
-
Gene ID number
7535
-
Tested applications
IF(IHC-P)
-
Recommended dilutions
IF(IHC-P)(1:50-200)
-
Crossreactivity
Human, Mouse, Rat
-
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Antigen background
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
-
Purification method
This antibody was purified via Protein A.
-
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
-
Excitation Emission
494nm/518nm
-
Synonyms
Zap-70Tyr493; ZAP70 phospho Y493; p-ZAP70 Y292; p-Zap-70Tyr292; p-Zap-70Y292; ZAP-70; ZAP 70; ZAP70; zeta-associated protein 70; ZAP-70=protein tyrosine kinase Syk homolog {SH2-like and C-terminal kinase domains}; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-associated protein; Syk-related tyrosine kinase. SRK; STD; TZK; ZAP70_HUMAN; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein.
-
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
-
Additional conjugation
Anti-FITC Antibody
-
French translation
anticorps
-
Gene target
-
Gene symbol
ZAP70
-
Short name
Anti-ZAP70 Tyr493
-
Technique
Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
-
Isotype
Immunoglobulin G (IgG)
-
Label
FITC
-
Alternative name
Anti-ZAP70 Tyr493 PAb FITC
-
Alternative technique
antibodies, fluorescine
-
Alternative to gene target
zeta-chain (TCR) associated protein kinase 70kDa, SRK and STCD and STD and TZK and ZAP-70, ZAP70 and IDBG-62509 and ENSG00000115085 and 7535, transferase activity, Cell surfaces, Zap70 and IDBG-145089 and ENSMUSG00000026117 and 22637, ZAP70 and IDBG-629842 and ENSBTAG00000005892 and 504509
-
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
-
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products