SHC (Tyr317) Antibody, FITC Conjugated

• Catalog number
  bs-3413R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  SHC (Tyr317) Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  SHC Tyr317
• Specificity
  This is a highly specific antibody against SHC Tyr317.
• Modification
  Phosphorylation
• Modification site
  Tyr317
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic phosphopeptide derived from human SHC around the phosphorylation site of Tyr317
• Gene ID number
  6464
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  The SHC gene encodes for a signaling and transforming protein containing Src homology 2 and 3 (SH2 and SH3) domains. The SHC gene encodes 2 widely expressed overlapping proteins of 46 and 52 kD, both containing a C-terminal SH2 domain. Adjacent to the SH2 region is a glycine and proline rich region. These 2 proteins differ in their N terminals. SHC proteins are involved in mitogenic signal transduction and act by coupling growth factor receptors to the RAS signaling pathway. The protein encoded by the SHC1 gene is thought to act as an adaptor in many signal transduction pathways.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  p46; p52; p52 SHC; p52SHC; p66; p66 SHC; p66SHC; SH2 domain protein C1; SHC Src homology 2 domain containing transforming protein 1; SHC 1; SHC A; Shc; SHC transforming protein 1; SHC transforming protein; SHC1; SHCA; Src homology 2 domain containing transforming protein C1.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  SHC   Tyr317   Conjugated  
• Gene symbol
  SHC1, SHC3
• Short name
  Anti-SHC Tyr317
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-SHC Tyr317 PAb FITC
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:10840
• Gene
  SHC1
• Long gene name
  SHC adaptor protein 1
• Synonyms gene
  • SHC
• Synonyms gene name
  • SHC (Src homology 2 domain-containing) transforming protein 1
• Synonyms
  • p66
  • ShcA
• GenBank acession
  • U73377
• Locus
  1q21.3
• Discovery year
  1993-02-17
• Entrez gene record
  6464
• Pubmed identfication
  • 1623525
• RefSeq identity
  • NM_183001
• Classification
  • SH2 domain containing
• VEGA ID
  OTTHUMG00000037295

Gene info

• Identity
  HGNC:18181
• Gene
  SHC3
• Long gene name
  SHC adaptor protein 3
• Synonyms gene name
  • src homology 2 domain containing transforming protein C3
  • SHC (Src homology 2 domain containing) transforming protein 3
• Synonyms
  • N-Shc
  • NSHC
  • SHCC
• GenBank acession
  • D84361
• Locus
  9q22.1
• Discovery year
  2003-11-12
• Entrez gene record
  53358
• Pubmed identfication
  • 8808684
• RefSeq identity
  • NM_016848
• Classification
  • SH2 domain containing
• VEGA ID
  OTTHUMG00000020179

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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