RPA2(Thr21) Antibody, Cy7 Conjugated
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Catalog number
bs-5693R-Cy7
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Price
Please ask
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Size
0.1ml
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Long name
RPA2(Thr21) Polyclonal Antibody, Cy7 Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
Cy7
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
RPA2Thr21
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Specificity
This is a highly specific antibody against RPA2Thr21.
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Modification
Phosphorylation
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Modification site
Thr21
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen_range
5-40/270
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Subcellular location
Nucleus
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Source
KLH conjugated synthetic phosphopeptide derived from human RPA2 around the phosphorylation site of Thr21
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Gene ID number
6118
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Swiss Prot
P15927
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
743nm/767nm
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Synonyms
REPA2; RPA32; RP-A p32; RP-A p34; Replication protein A 32 kDa subunit; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RPA2; RPA34
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugated
These antibodies are excite for emission at 650 nm and detected at a 676 nm wavelengths.
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Additional conjugation
Cy7
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French translation
anticorps
Gene info
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Identity
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Gene
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Long gene name
replication protein A2
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Synonyms gene name
- replication protein A2 (32kD)
- replication protein A2, 32kDa
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GenBank acession
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Locus
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Discovery year
1993-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Nucleotide excision repair
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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