PRRSV Antibody, FITC Conjugated
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Catalog number
bs-10043R-FITC
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Price
Please ask
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Size
0.1ml
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Long name
PRRSV Polyclonal Antibody, FITC Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
FITC
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
PRRSV
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Specificity
This is a highly specific antibody against PRRSV.
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Modification
Unmodified
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Modification site
None
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Source
Derived from an inactive PRRSV vaccine strain NVDC-JXA1
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Virus
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Crossreactive species details
PRRSV
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Antigen background
PRRSV belongs to the family Arteriviridae in the genus Arterivirus, order Nidovirales. This group also includes lactate dehydrogenase-elevating virus (LDV) of mice, equine arteritis virus (EAV) and simian hemorrhagic fever virus (SHFV). PRRSV is a small, enveloped virus of 45-70 nm in diameter. Its genome consists of a polyadenylated, single-stranded, non-segmented, positivesense RNA polycystronic molecule of 15.1-15.5 kb. It contains, in the 5’ to 3’ direction, a 5’ leader sequence, at least 10 open reading frames (ORF1a, ORF1b and ORFs 2a, 2b, 3, 4, 5, 5a, 6 and 7) and a 3’ non-translated region. ORF1a and ORF1b are located directly downstream of the 5’ leader and encode a large replicase polyprotein, which is thought to be autoproteolytically cleaved into at least 13 smaller non-structural proteins including a viral RNA-dependent RNA polymerase.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
494nm/518nm
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Synonyms
Porcine reproductive and respiratory syndrome, PRRS.
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
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Additional conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Short name
Anti-PRRSV
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
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Isotype
Immunoglobulin G (IgG)
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Label
FITC
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Alternative name
Anti-PRRSV PAb FITC
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Alternative technique
antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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