PAK3 (Ser139) Antibody, FITC Conjugated

• Catalog number
  bs-1655R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  PAK3 (Ser139) Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  PAK3 Ser139
• Specificity
  This is a highly specific antibody against PAK3 Ser139.
• Modification
  Phosphorylation
• Modification site
  Ser139
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic phosphopeptide derived from human PAK3 around the phosphorylation site of (Ser139)
• Gene ID number
  5063
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  PAK3_HUMAN; Beta PAK; bPAK; CDKN1A; hPAK3; Mental retardation X linked 30; MRX30; MRX47; Oligophrenin 3; OPHN3; p21 activated kinase 3; p21 CDKN1A activated kinase 3; PAK-3; PAK3 p21 protein Cdc42/Rac-activated kinase 3; PAK3beta; Pak65alpha; Pak65beta; Serine threonine protein kinase PAK 3; Stk4.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  PAK3   Ser139   Conjugated  
• Gene symbol
  PAK3, ARHGEF7
• Short name
  Anti-PAK3 Ser139
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-PAK3 Ser139 PAb FITC
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:8592
• Gene
  PAK3
• Long gene name
  p21 (RAC1) activated kinase 3
• Synonyms gene
  • MRX30
  • MRX47
• Synonyms gene name
  • mental retardation, X-linked 47
  • p21 (CDKN1A)-activated kinase 3
  • p21 protein (Cdc42/Rac)-activated kinase 3
• Synonyms
  • hPAK3
  • bPAK
• GenBank acession
  • AF068864
• Locus
  Xq23
• Discovery year
  1998-03-25
• Entrez gene record
  5063
• Pubmed identfication
  • 8826460
  • 9731525
• RefSeq identity
  • NM_002578
• VEGA ID
  OTTHUMG00000022202

Gene info

• Identity
  HGNC:15607
• Gene
  ARHGEF7
• Long gene name
  Rho guanine nucleotide exchange factor 7
• Synonyms gene name
  • Rho guanine nucleotide exchange factor (GEF) 7
• Synonyms
  • KIAA0142
  • PIXB
  • DKFZp761K1021
  • Nbla10314
  • DKFZp686C12170
  • BETA-PIX
  • COOL1
  • P85SPR
  • P85
  • P85COOL1
  • P50BP
  • PAK3
  • P50
• Synonyms name
  • SH3 domain-containing proline-rich protein
  • PAK-interacting exchange factor beta
• GenBank acession
  • D63476
• Locus
  13q34
• Discovery year
  2001-11-21
• Entrez gene record
  8874
• Pubmed identfication
  • 9207241
  • 9726964
• RefSeq identity
  • NM_001113511
• Classification
  • Pleckstrin homology domain containing
  • Dbl family Rho GEFs
• VEGA ID
  OTTHUMG00000017357

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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