PAK1(Thr212) Antibody, FITC Conjugated

• Catalog number
  bs-5542R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  PAK1(Thr212) Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  PAK1 Thr212
• Specificity
  This is a highly specific antibody against PAK1 Thr212.
• Modification
  Phosphorylation
• Modification site
  Thr212
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of Thr212
• Gene ID number
  5058
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  PAK1Thr212; PAK1phospho T212; Alpha PAK; Alpha-PAK; MGC130000; MGC130001; p21 activated kinase 1; p21 protein Cdc42/Rac activated kinase 1; p21-activated kinase 1; p21/Cdc42/Rac1 activated kinase 1 yeast Ste20 related; p21/Cdc42/Rac1 activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 STE20 homolog, yeast; p65 PAK; p65-PAK; PAK alpha; PAK-1; Pak1; PAK1_HUMAN; Paka; PAKalpha; Protein kinase MUK2; Rac/p21-activated kinase; Serine/threonine-protein kinase PAK 1; STE20 homolog yeast; STE20 homolog yeast.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  PAK1   Thr212   Conjugated  
• Gene symbol
  PAK1, PKN1
• Short name
  Anti-PAK1 Thr212
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-PAK1 Thr212 PAb FITC
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  p21 protein (Cdc42/Rac)-activated kinase 1, PAKalpha, PAK1 and IDBG-65610 and ENSG00000149269 and 5058, transferase activity, nuclei, Pak1 and IDBG-199974 and ENSMUSG00000030774 and 18479, PAK1 and IDBG-642220 and ENSBTAG00000010191 and 533729

Gene info

• Identity
  HGNC:8590
• Gene
  PAK1
• Long gene name
  p21 (RAC1) activated kinase 1
• Synonyms gene name
  • p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
  • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
  • p21 protein (Cdc42/Rac)-activated kinase 1
• Synonyms name
  • STE20 homolog, yeast
• GenBank acession
  • U51120
• Locus
  11q13.5-q14.1
• Discovery year
  1997-12-05
• Entrez gene record
  5058
• Pubmed identfication
  • 8805275
  • 9533029
• RefSeq identity
  • NM_002576
• VEGA ID
  OTTHUMG00000165138

Gene info

• Identity
  HGNC:9405
• Gene
  PKN1
• Long gene name
  protein kinase N1
• Synonyms gene
  • PRKCL1
• Synonyms gene name
  • protein kinase C-like 1
• Synonyms
  • DBK
  • PRK1
  • PKN
  • MGC46204
  • PAK1
• GenBank acession
  • S75546
• Locus
  19p13.12
• Discovery year
  1997-10-10
• Entrez gene record
  5585
• Pubmed identfication
  • 9570957
• RefSeq identity
  • NM_002741
  • NM_213560
• Classification
  • C2 domain containing protein kinases
  • AGC family kinases
• VEGA ID
  OTTHUMG00000039611

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products