OTX2 Antibody, FITC Conjugated

• Catalog number
  bs-11597R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  OTX2 Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  OTX2
• Specificity
  This is a highly specific antibody against OTX2.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic peptide derived from human OTX2
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Transcription factors, OTX1 and OTX2, are two murine homologs of the Drosophila orthodenticle (OTD), show a limited amino acid sequence divergence. OTX1 and OTX2 play an important role during early and later events required for proper brain development in that they are involved in the processes of induction, specification and regionalization of the brain. OTX1 is involved in corticogenesis, sensory organ development and pituitary functions, while OTX2 is necessary earlier in development, for the correct anterior neural plate specification and organization of the primitive streak. OTX2 is also required in the early specification of the neuroectoderm, which is destined to become the fore-midbrain, and both OTX1 and OTX2 co-operate in patterning the developing brain through a dosage-dependent mechanism. A molecular mechanism depending on a precise threshold of OTX proteins is necessary for the correct positioning of the isthmic region and for anterior brain patterning. The genes which encode OTX1 and OTX2 map to human chromosomes 2p13 and 14q21-q22, respectively.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  CPHD6; Homeobox protein OTX2; MCOPS 5; MCOPS5; MGC45000; Orthodenticle 2; Orthodenticle homeobox 2; Orthodenticle homolog 2 Drosophila; Orthodenticle homolog 2; Orthodenticle2; Otx 2; otx2; OTX2_HUMAN.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  OTX2   Conjugated  
• Gene symbol
  OTX2, OTX2-AS1
• Short name
  Anti-OTX2
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-OTX2 PAb FITC
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:8522
• Gene
  OTX2
• Long gene name
  orthodenticle homeobox 2
• Synonyms gene name
  • orthodenticle homolog 2 (Drosophila)
• GenBank acession
  • AF298117
• Locus
  14q22.3
• Discovery year
  1994-02-08
• Entrez gene record
  5015
• Pubmed identfication
  • 7959790
• RefSeq identity
  • NM_021728.
• Classification
  • PRD class homeoboxes and pseudogenes
• VEGA ID
  OTTHUMG00000152338
• Locus Specific Databases
  • LOVD
  • LRG_718

Gene info

• Identity
  HGNC:43906
• Gene
  OTX2-AS1
• Long gene name
  OTX2 antisense RNA 1 (head to head)
• Synonyms gene name
  • OTX2 antisense RNA 1 (non-protein coding)
  • OTX2 antisense RNA 1
• Synonyms
  • OTX2OS1
• GenBank acession
  • BC041486
• Locus
  14q22.3
• Discovery year
  2012-05-01
• Entrez gene record
  100309464
• Pubmed identfication
  • 15703187
• RefSeq identity
  • NR_029385
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000171304

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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