NFAT5 (Ser1197) Antibody, Biotin Conjugated

• Catalog number
  bs-9474R-Biotin
• Price
  Please ask
• Size
  0.1ml

• Long name
  NFAT5 (Ser1197) Polyclonal Antibody, Biotin Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  Biotin
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  NFAT5 Ser1197
• Specificity
  This is a highly specific antibody against NFAT5 Ser1197.
• Modification
  Phosphorylation
• Modification site
  Ser1197
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic phosphopeptide derived from human NFAT5 around the phosphorylation site of Ser1197
• Gene ID number
  10725
• Tested applications
  WB, IHC-P
• Recommended dilutions
  WB(1:100-1000), IHC-P(1:100-500)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Members of the NFAT (nuclear factor of activated T cells) family of transcription factors are related to NFkB/Rel proteins and form cooperative complexes with the AP-1 proteins, Fos and Jun, on DNA to regulate cytokine expression in T cells. NFAT proteins are widely expressed and alternatively modified to generate splice variants, and they are localized to both the cytosol (NFATc) and to the nucleus (NFATn). NFAT1, NFAT2, and NFAT4 are predominantly expressed in immune cells, and NFAT2 and NFAT3 are expressed at high levels in cardiac tissues. In addition to activating cytokine gene transcription, NFAT2 is also implicated in cardiac valve development, and NFAT3 is involved in cardiac hypertrophy. NFAT5 is detected in both immune and nonimmune cells and, like other NFAT proteins, contains a highly conserved Rel-like binding domain that mediates NFAT proteins associating with specific consensus sequences on DNA. NFAT proteins are activated by increases in intracellular calcium, which leads to the calmodulin-dependent phosphatase, calcineurin, dephosphorylating NFAT proteins. This activating event induces a conformational change in the protein structure that exposes the nuclear localization signal and facilitates the translocation of NFAT proteins from the cytosol into the nucleus.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  N/A
• Synonyms
  NFAT5 phospho S1197; p-NFAT5phospho S1197; Glutamine rich protein H65; KIAA0827; NF AT5; NF-AT5; NFAT 5; NFAT L1; NFAT like protein 1; NFAT5; NFAT5_HUMAN; NFATL 1; NFATL1; NFATZ; Nuclear factor of activated T cells 5; Nuclear factor of activated T cells 5 tonicity responsive; Nuclear factor of activated T cells; Nuclear factor of activated T-cells 5; OREBP; Osmotic response element binding protein; T cell transcription factor NFAT 5; T cell transcription factor NFAT5; T-cell transcription factor NFAT5; TonE binding protein; TonE-binding protein ; TonEBP; Tonicity responsive enhancer binding protein; Tonicity-responsive enhancer-binding protein.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Additional conjugation
  Biotinylated
• French translation
  anticorps

• Gene target
  NFAT5   Ser1197   Conjugated  
• Gene symbol
  NFAT5
• Short name
  Anti-NFAT5 Ser1197
• Technique
  Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
• Isotype
  Immunoglobulin G (IgG)
• Label
  Biotin
• Alternative name
  Anti-NFAT5 Ser1197 PAb Biotin
• Alternative technique
  antibodies
• Alternative to gene target
  nuclear factor of activated T-cells 5, tonicity-responsive, NF-AT5 and NFATL1 and NFATZ and OREBP and TONEBP, NFAT5 and IDBG-39265 and ENSG00000102908 and 10725, protein binding, nuclei, Nfat5 and IDBG-190689 and ENSMUSG00000003847 and 54446, NFAT5 and IDBG-634541 and ENSBTAG00000013412 and 538523

Gene info

• Identity
  HGNC:7774
• Gene
  NFAT5
• Long gene name
  nuclear factor of activated T cells 5
• Synonyms gene name
  • nuclear factor of activated T-cells 5, tonicity-responsive
  • nuclear factor of activated T-cells 5
• Synonyms
  • TONEBP
  • KIAA0827
  • NFATL1
  • OREBP
  • NFATZ
  • NF-AT5
• Synonyms name
  • tonicity-responsive enhancer binding protein
• GenBank acession
  • AF134870
• Locus
  16q22.1
• Discovery year
  1999-07-16
• Entrez gene record
  10725
• Pubmed identfication
  • 10377394
• RefSeq identity
  • NM_138714
• Classification
  • IPT domain containing
  • Nuclear factors of activated T-cells
• VEGA ID
  OTTHUMG00000137572
• Locus Specific Databases
  • LRG_1332

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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