NEDD4L (Ser342) Antibody, FITC Conjugated

• Catalog number
  bs-3299R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  NEDD4L (Ser342) Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  NEDD4L Ser342
• Specificity
  This is a highly specific antibody against NEDD4L Ser342.
• Modification
  Phosphorylation
• Modification site
  Ser342
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic phosphopeptide derived from human NEDD4L around the phosphorylation site of Ser342
• Gene ID number
  23327
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  E3 ubiquitin-protein ligase accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Furthermore, it promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5, and promotes the ubiquitination and degradation of SGK.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  E3 ubiquitin protein ligase NEDD4 like protein; E3 ubiquitin-protein ligase NEDD4-like; E3 ubiquitin-protein ligase NEDD4-like protein; KIAA0439; NED4L_HUMAN; NEDD4 2; NEDD4 2c; NEDD4-2; NEDD4-2a; NEDD4.2; NEDD4b; NEDD4L; NEDD4La; NEDD4Lb; NEDD4Lc; NEDD4Ld; NEDD4Le; NEDD4Lf; NEDD4Lg; NEDD4Lh; NEDL3; Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase; Neural precursor cell expressed, developmentally down regulated 4 like; RSP5; Ubiquitin protein ligase Rsp5.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  NEDD4L   Ser342   Conjugated  
• Gene symbol
  NEDD4L
• Short name
  Anti-NEDD4L Ser342
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-NEDD4L Ser342 PAb FITC
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase, hNEDD4-2 and NEDD4-2 and NEDD4.2 and RSP5, NEDD4L and IDBG-3835 and ENSG00000049759 and 23327, ion channel binding, nuclei, Nedd4l and IDBG-154290 and ENSMUSG00000024589 and 83814, NEDD4L and IDBG-644828 and ENSBTAG00000005412 and 510003

Gene info

• Identity
  HGNC:7728
• Gene
  NEDD4L
• Long gene name
  NEDD4 like E3 ubiquitin protein ligase
• Synonyms gene name
  • neural precursor cell expressed, developmentally down-regulated 4-like
  • neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase
• Synonyms
  • KIAA0439
  • RSP5
  • NEDD4-2
• GenBank acession
  • AF210730
• Locus
  18q21.31
• Discovery year
  2000-03-14
• Entrez gene record
  23327
• Pubmed identfication
  • 10594025
  • 11244092
  • 18322022
• Classification
  • HECT domain containing
  • C2 domain containing
• VEGA ID
  OTTHUMG00000179875

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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