Nanog Antibody, Biotin Conjugated

• Catalog number
  bs-10414R-Biotin
• Price
  Please ask
• Size
  0.1ml

• Long name
  Nanog Polyclonal Antibody, Biotin Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  Biotin
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  Nanog
• Specificity
  This is a highly specific antibody against Nanog.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  140-175/305
• Subcellular location
  Nucleus
• Source
  KLH conjugated synthetic peptide derived from mouse Nanog
• Gene ID number
  71950
• Swiss Prot
  Q80Z64
• Tested applications
  IHC-P
• Recommended dilutions
  IHC-P(1:100-500)
• Crossreactivity
  Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  N/A
• Synonyms
  ENK; ecat4; 2410002E02Rik; Homeobox protein NANOG; ES cell-associated protein 4; Early embryo specific expression NK-type homeobox protein; Homeobox transcription factor Nanog; Nanog
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Additional conjugation
  Biotinylated
• French translation
  anticorps

• Gene target
  Nanog   Conjugated  
• Short name
  Anti-Nanog
• Technique
  Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
• Isotype
  Immunoglobulin G (IgG)
• Label
  Biotin
• Alternative name
  Anti-Nanog PAb Biotin
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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