MYPT1 (Thr853) Antibody, Biotin Conjugated

• Catalog number
  bs-3288R-Biotin
• Price
  Please ask
• Size
  0.1ml

• Long name
  MYPT1 (Thr853) Polyclonal Antibody, Biotin Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  Biotin
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  MYPT1 Thr853
• Specificity
  This is a highly specific antibody against MYPT1 Thr853. This phosphorylation site is homologous to that of Thr852 in Mouse and Thr855 in Rat.
• Modification
  Phosphorylation
• Modification site
  Thr853
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  830-880/1030
• Subcellular location
  Cytoplasm
• Source
  KLH conjugated synthetic phosphopeptide derived from human around the phosphorylation site of Thr853
• Gene ID number
  4659
• Swiss Prot
  O14974
• Tested applications
  WB, IHC-P
• Recommended dilutions
  WB(1:100-1000), IHC-P(1:100-500)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  N/A
• Synonyms
  MBS; M130; MYPT1; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit; PPP1R12A
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Additional conjugation
  Biotinylated
• French translation
  anticorps

• Gene target
  MYPT1   Thr853   Conjugated  
• Gene symbol
  PPP1R12A
• Short name
  Anti-MYPT1 Thr853
• Technique
  Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
• Isotype
  Immunoglobulin G (IgG)
• Label
  Biotin
• Alternative name
  Anti-MYPT1 Thr853 PAb Biotin
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:7618
• Gene
  PPP1R12A
• Long gene name
  protein phosphatase 1 regulatory subunit 12A
• Synonyms gene
  • MYPT1
• Synonyms gene name
  • protein phosphatase 1, regulatory (inhibitor) subunit 12A
  • protein phosphatase 1, regulatory subunit 12A
• Synonyms
  • MBS
  • M130
• Synonyms name
  • myosin phosphatase-targeting subunit 1
  • myosin binding subunit
• GenBank acession
  • D87930
• Locus
  12q21.2-q21.31
• Discovery year
  1997-12-23
• Entrez gene record
  4659
• Pubmed identfication
  • 9286714
• RefSeq identity
  • NM_002480
• Classification
  • Ankyrin repeat domain containing
  • Myosin phosphatase targeting family
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000170100

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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