MSH6 Antibody, FITC Conjugated

• Catalog number
  bs-3804R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  MSH6 Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  MSH6
• Specificity
  This is a highly specific antibody against MSH6.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  830-880/1360
• Subcellular location
  Nucleus
• Source
  KLH conjugated synthetic peptide derived from human MSH6
• Gene ID number
  2956
• Swiss Prot
  P52701
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  GTBP; HSAP; p160; GTMBP; HNPCC5; DNA mismatch repair protein Msh6; hMSH6; G/T mismatch-binding protein; MutS-alpha 160 kDa subunit; MSH6
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  MSH6   Conjugated  
• Gene symbol
  MSH6
• Short name
  Anti-MSH6
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-MSH6 PAb FITC
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  mutS homolog 6 (E. coli), GTBP and GTMBP and HNPCC5 and HSAP and p160, MSH6 and IDBG-51044 and ENSG00000116062 and 2956, ADP binding, nuclei, Msh6 and IDBG-201548 and ENSMUSG00000005370 and 17688, BT.98842 and IDBG-633641 and ENSBTAG00000001424 and 540526

Gene info

• Identity
  HGNC:7329
• Gene
  MSH6
• Long gene name
  mutS homolog 6
• Synonyms gene
  • GTBP
• Synonyms gene name
  • mutS (E. coli) homolog 6
  • mutS homolog 6 (E. coli)
• GenBank acession
  • U54777
• Locus
  2p16.3
• Discovery year
  1995-08-29
• Entrez gene record
  2956
• Pubmed identfication
  • 7604266
• RefSeq identity
  • NM_000179
• Classification
  • MutS homologs
  • PWWP domain containing
• VEGA ID
  OTTHUMG00000129129
• Locus Specific Databases
  • Hereditary Non-Polyposis Colorectal Cancer, HNPCC
  • Global Variome shared LOVD
  • MSH6 database at LOVD-China
  • LRG_219

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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