MLH1 Antibody, Biotin Conjugated
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Catalog number
bs-0836R-Biotin
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Price
Please ask
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Size
0.1ml
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Long name
MLH1 Polyclonal Antibody, Biotin Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
Biotin
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
MLH1
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Specificity
This is a highly specific antibody against MLH1.
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Modification
Unmodified
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Modification site
None
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen_range
520-570/756
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Subcellular location
Nucleus
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Source
KLH conjugated synthetic peptide derived from human MLH1
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Gene ID number
4292
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Swiss Prot
P40692
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Tested applications
WB, IHC-P
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Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500)
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Crossreactivity
Human, Mouse, Rat
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
N/A
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Synonyms
FCC2; COCA2; HNPCC; hMLH1; HNPCC2; DNA mismatch repair protein Mlh1; MutL protein homolog 1; MLH1
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Additional conjugation
Biotinylated
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French translation
anticorps
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Gene target
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Gene symbol
MLH1
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Short name
Anti-MLH1
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Technique
Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
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Isotype
Immunoglobulin G (IgG)
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Label
Biotin
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Alternative name
Anti-MLH1 PAb Biotin
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Alternative technique
antibodies
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Alternative to gene target
mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli), COCA2 and FCC2 and hMLH1 and HNPCC and HNPCC2, MLH1 and IDBG-24600 and ENSG00000076242 and 4292, structure-specific DNA binding, nuclei, Mlh1 and IDBG-202755 and ENSMUSG00000032498 and 17350, MLH1 and IDBG-644253 and ENSBTAG00000016758 and 533652
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Gene info
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Identity
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Gene
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Long gene name
mutL homolog 1
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Synonyms gene
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Synonyms gene name
- mutL (E. coli) homolog 1 (colon cancer, nonpolyposis type 2)
- mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
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Synonyms
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GenBank acession
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Locus
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Discovery year
1993-11-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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