MAPKAPK5(Thr182) Antibody, Biotin Conjugated
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Catalog number
bs-5504R-Biotin
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Price
Please ask
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Size
0.1ml
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Long name
MAPKAPK5(Thr182) Polyclonal Antibody, Biotin Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
Biotin
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
MAPKAPK5 Thr182
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Specificity
This is a highly specific antibody against MAPKAPK5 Thr182. This phosphorylation site is homologous to that of Thr182 in Mouse.
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Modification
Phosphorylation
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Modification site
Thr182
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen_range
160-210/473
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Subcellular location
Cytoplasm, Nucleus
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Source
KLH conjugated synthetic phosphopeptide derived from human MAPKAPK5 around the phosphorylation site of Thr182
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Gene ID number
8550
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Swiss Prot
Q8IW41
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Tested applications
WB, IHC-P
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Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500)
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Crossreactivity
Human, Mouse
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
N/A
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Synonyms
MK5; MK-5; PRAK; MAPKAP-K5; MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAPK-5; p38-regulated/activated protein kinase; MAPKAPK5
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Additional conjugation
Biotinylated
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French translation
anticorps
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Gene target
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Gene symbol
MAPKAPK5-AS1, MAPKAPK5
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Short name
Anti-MAPKAPK5 Thr182
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Technique
Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
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Isotype
Immunoglobulin G (IgG)
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Label
Biotin
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Alternative name
Anti-MAPKAPK5 Thr182 PAb Biotin
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Alternative technique
antibodies
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Gene info
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Identity
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Gene
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Long gene name
MAPKAPK5 antisense RNA 1
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Synonyms gene
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Synonyms gene name
- chromosome 12 open reading frame 47
- MAPKAPK5 antisense RNA 1 (non-protein coding)
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Synonyms
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Locus
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Discovery year
2006-01-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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