MAPK8 (Thr183 + Tyr185) Antibody, FITC Conjugated
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Catalog number
bs-5487R-FITC
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Price
Please ask
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Size
0.1ml
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Long name
MAPK8 (Thr183 + Tyr185) Antibody, FITC Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
FITC
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
MAPK8 Thr183 + Tyr185
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Specificity
This is a highly specific antibody against MAPK8 Thr183 + Tyr185.
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Modification
Phosphorylation
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Modification site
Thr183 + Tyr185
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Source
KLH conjugated synthetic phosphopeptide derived from human MAPK9 around the phosphorylation site of Thr183+Tyr185
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Gene ID number
5601
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
phosphorylated at the Thr-Pro-Tyr phosphorylation motif instead of the characteristic MAP kinase Thr-Glu-Tyr motif. JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
494nm/518nm
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Synonyms
MAPK8/MAPK9phospho T183/Y185; Mapk9; C Jun kinase 2; C Jun N terminal kinase 2; JNK 2; JNK-2; JNK 55; JNK2 alpha; JNK2 beta; JNK2alpha; JNK2B; JNK2beta; JNK55; Jun kinase; MAP kinase 9; MAPK 9; Mitogen activated protein kinase 9; P54 alpha; P54aSAPK; PRKM9; SAPK alpha; SAPK1a; SAPKa; Stress activated protein kinase JNK2.
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
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Additional conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Gene symbol
MAPK8
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Short name
Anti-MAPK8 Thr183 + Tyr185
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
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Isotype
Immunoglobulin G (IgG)
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Label
FITC
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Alternative name
Anti-MAPK8 Thr183 + Tyr185 PAb FITC
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Alternative technique
antibodies, fluorescine
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Alternative to gene target
mitogen-activated protein kinase 8, JNK and JNK-46 and JNK1 and JNK1A2 and JNK21B1/2 and PRKM8 and SAPK1 and SAPK1c, MAPK8 and IDBG-73479 and ENSG00000107643 and 5599, transferase activity, nuclei, Mapk8 and IDBG-148417 and ENSMUSG00000021936 and 26419, MAPK8 and IDBG-631635 and ENSBTAG00000007876 and 539941
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Gene info
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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