MAPK3(Tyr204) Antibody, FITC Conjugated

• Catalog number
  bs-5497R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  MAPK3(Tyr204) Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  MAPK3 Tyr204
• Specificity
  This is a highly specific antibody against MAPK3 Tyr204.
• Modification
  Phosphorylation
• Modification site
  Tyr204
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic phosphopeptide derived from human MAPK3 around the phosphorylation site of Tyr204
• Gene ID number
  5595
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  MAPK3phospho Y204; ERK/MAPKphospho T202/Y204; p44/42 MAP KinaseThr202; ERK 1; ERK; ERK-1; ERK1; Extracellular Signal Regulated Kinase 1; Extracellular signal related kinase 1; Extracellular signal-regulated kinase 1; HGNC6877; HS44KDAP; HUMKER1A; MAP kinase 1; MK03_RAT; MAP Kinase; MAP kinase isoform p44; MAPK 1; MAPK; MAPK1; MAPK3; MGC20180; Mitogen-activated protein kinase 1; OTTHUMP00000174538; OTTHUMP00000174541; p44 ERK1; p44 MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK;
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  MAPK3   Tyr204   Conjugated  
• Gene symbol
  MAPK3
• Short name
  Anti-MAPK3 Tyr204
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-MAPK3 Tyr204 PAb FITC
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  mitogen-activated protein kinase 3, ERK-1 and ERK1 and ERT2 and HS44KDAP and HUMKER1A and p44-ERK1 and p44-MAPK and P44ERK1 and P44MAPK and PRKM3, MAPK3 and IDBG-25745 and ENSG00000102882 and 5595, transferase activity, nuclei, Mapk3 and IDBG-209781 and ENSMUSG00000063065 and 26417, MAPK3 and IDBG-639178 and ENSBTAG00000016156 and 531391

Gene info

• Identity
  HGNC:6877
• Gene
  MAPK3
• Long gene name
  mitogen-activated protein kinase 3
• Synonyms gene
  • PRKM3
• Synonyms
  • ERK1
  • p44mapk
  • p44erk1
• GenBank acession
  • M84490
• Locus
  16p11.2
• Discovery year
  1993-11-05
• Entrez gene record
  5595
• Pubmed identfication
  • 9628824
• Classification
  • Mitogen-activated protein kinases
• VEGA ID
  OTTHUMG00000132149

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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