LCAT Antibody, FITC Conjugated
#
-
Catalog numberbs-1972R-FITC
-
Price:
-
Size0.1ml
-
-
Long nameLCAT Polyclonal Antibody, FITC Conjugated
-
CategoryConjugated Primary Antibodies
-
ConjugationFITC
-
Host OrganismRabbit (Oryctolagus cuniculus)
-
Target AntigenLCAT
-
SpecificityThis is a highly specific antibody against LCAT.
-
ModificationUnmodified
-
Modification siteNone
-
ClonalityPolyclonal
-
ClonePolyclonal antibody
-
Concentration1ug per 1ul
-
Immunogen_range170-220/440
-
Subcellular locationSecreted
-
SourceKLH conjugated synthetic peptide derived from human LCAT
-
Gene ID number3931
-
Swiss ProtP04180
-
Tested applicationsIF(IHC-P)
-
Recommended dilutionsIF(IHC-P)(1:50-200)
-
CrossreactivityHuman, Mouse, Rat
-
Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Antigen backgroundCentral enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.
-
Purification methodThis antibody was purified via Protein A.
-
Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
-
Excitation Emission494nm/518nm
-
SynonymsPhosphatidylcholine-sterol acyltransferase; Lecithin-cholesterol acyltransferase; Phospholipid-cholesterol acyltransferase; LCAT
-
PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
-
Additional conjugationAnti-FITC Antibody
-
French translationanticorps
-
Gene target
-
Gene symbolLCAT
-
Short nameAnti-LCAT
-
TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
-
IsotypeImmunoglobulin G (IgG)
-
LabelFITC
-
Alternative nameAnti-LCAT PAb FITC
-
Alternative techniqueantibodies, fluorescine
-
Gene info
-
Identity
-
Gene
-
Long gene namelecithin-cholesterol acyltransferase
-
Synonyms name
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
-
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data