JNK1+2+3 (T183+Y185) Antibody, Cy3 Conjugated
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Catalog number
bs-1640R-Cy3
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Price
Please ask
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Size
0.1ml
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Long name
JNK1+2+3 (T183+Y185) Polyclonal Antibody, Cy3 Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
Cy3
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
JNK1+2+3 (THR183+TYR185)
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Specificity
This is a highly specific antibody against JNK1+2+3 (THR183+TYR185).
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Modification
Phosphorylation
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Modification site
THR183+TYR185
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen_range
170-210/427
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Subcellular location
Cytoplasm, Nucleus
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Source
KLH conjugated synthetic phosphopeptide derived from human JNK1 around the phosphorylation site of Thr183/Tyr185
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Gene ID number
5599
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Swiss Prot
P45983
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat, Bovine, Dog
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
512,550nm/570,615nm
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Synonyms
JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2; Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; MAPK8
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-JNK1+2+3 (THR183+TYR185) can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
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Additional conjugation
cy3 conjugation kit
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French translation
anticorps
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Gene target
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Gene symbol
MAPK8
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Short name
Anti-JNK1+2+3 (THR183+TYR185)
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Technique
Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
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Isotype
Immunoglobulin G (IgG)
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Label
Cy3
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Alternative name
Anti-JNK1+2+3 (THR183+TYR185) PAb Cy3
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Alternative technique
antibodies
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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