FHIT (Tyr114) Antibody, FITC Conjugated

• Catalog number
  bs-3146R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  FHIT (Tyr114) Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  FHIT Tyr114
• Specificity
  This is a highly specific antibody against FHIT Tyr114.
• Modification
  Phosphorylation
• Modification site
  Tyr114
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  90-130/147
• Subcellular location
  Cytoplasm, Nucleus, Mitochondrion
• Source
  KLH conjugated synthetic phosphopeptide derived from human FHIT around the phosphorylation site of Tyr114
• Gene ID number
  2272
• Swiss Prot
  P49789
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  FRA3B; AP3Aase; Bis(5'-adenosyl)-triphosphatase; AP3A hydrolase; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein; FHIT
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  FHIT   Tyr114   Conjugated  
• Gene symbol
  FHIT
• Short name
  Anti-FHIT Tyr114
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-FHIT Tyr114 PAb FITC
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:3701
• Gene
  FHIT
• Long gene name
  fragile histidine triad diadenosine triphosphatase
• Synonyms gene name
  • fragile histidine triad gene
  • fragile histidine triad
• Synonyms
  • FRA3B
  • AP3Aase
• Synonyms name
  • bis(5'-adenosyl)-triphosphatase
• GenBank acession
  • BC032336
• Locus
  3p14.2
• Discovery year
  1996-10-18
• Entrez gene record
  2272
• Pubmed identfication
  • 8598045
  • 9671749
  • 26181368
• RefSeq identity
  • NM_002012
• Classification
  • Histidine triad superfamily
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000158591

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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