EYA2 Antibody, FITC Conjugated

• Catalog number
  bs-13125R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  EYA2 Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  EYA2
• Specificity
  This is a highly specific antibody against EYA2.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic peptide derived from human EYA2
• Gene ID number
  2139
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  A gene on chromosome 20q13.1 encodes Eya2 (eyes absent). EYA2 is one of four members of the eyes absent family. A 271 amino acid domain at the carboxy-terminal is highly conserved amongst the members of the eyes absent family, while the PST (proline-serive-threonin)-rich amino-terminal is highly divergent. EYA2 is expressed relatively late in development in the cytoplasm of extensor tendons and ligaments of the phalangeal elements of the limb, cranial placodes, branchial arches, central nervous system, and the developing eye. Pax3 induces the expression of Eya2 in a cascade that is necessary and sufficient for myogenesis. EYA2, like EYA1, acts as a transcriptional activator in connective tissue patterning through its PST domain, which functions as a transactivation domain. EYA2 is translocated to the nucleus by Six proteins, which interact through their domain and homeodomain with EYA2. EYA2 carboxy-terminal interacts with the G Alpha z and G Alphai 2 proteins. This interaction prevents Six proteins from translocating EYA2 to the nucleus.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  EAB 1; EAB1; EYA 2; EYA2; EYA2_HUMAN; eyes absent 2 homolog Drosophila; Eyes absent homolog 2; Translation of this uORF probably lowers the translation efficiency of EYA2.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  EYA2   Conjugated  
• Gene symbol
  EYA2
• Short name
  Anti-EYA2
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-EYA2 PAb FITC
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  eyes absent homolog 2 (Drosophila), EAB1, EYA2 and IDBG-79457 and ENSG00000064655 and 2139, protein binding, nuclei, Eya2 and IDBG-212752 and ENSMUSG00000017897 and 14049, EYA2 and IDBG-643097 and ENSBTAG00000013336 and 615264

Gene info

• Identity
  HGNC:3520
• Gene
  EYA2
• Long gene name
  EYA transcriptional coactivator and phosphatase 2
• Synonyms gene name
  • eyes absent (Drosophila) homolog 2
  • eyes absent homolog 2 (Drosophila)
• Synonyms
  • EAB1
• Locus
  20q13.12
• Discovery year
  1997-06-24
• Entrez gene record
  2139
• Pubmed identfication
  • 9020840
• RefSeq identity
  • NM_005244
• Classification
  • EYA transcriptional coactivator and phosphatases
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000033041

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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