Ephrin B (Tyr324 + Tyr329) Antibody, FITC Conjugated

• Catalog number
  bs-3129R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  Ephrin B (Tyr324 + Tyr329) Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  Ephrin B Tyr324 + Tyr329
• Specificity
  This is a highly specific antibody against Ephrin B Tyr324 + Tyr329. These phosphorylation sites are homologous to Tyr323 + Tyr328 in Mouse.
• Modification
  Phosphorylation
• Modification site
  Tyr324 + Tyr329
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  300-346/346
• Subcellular location
  Cytoplasm, Cell membrane
• Source
  KLH conjugated synthetic phosphopeptide derived from human Ephrin B around the phosphorylation site of Tyr324/329
• Gene ID number
  1947
• Swiss Prot
  P98172
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity). Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to the receptor tyrosine kinases EPHB3 (preferred), EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2; Ephrin-B1; EFL-3; ELK ligand; EPH-related receptor tyrosine kinase ligand 2; LERK-2; EFNB1
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  Ephrin   Tyr324   Tyr329   Conjugated  
• Short name
  Anti-Ephrin B Tyr324 + Tyr329
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-Ephrin B Tyr324 + Tyr329 PAb FITC
• Alternative technique
  antibodies, fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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